Comparison of Genome Sequence and Biological Properties of Broad bean wilt virus 2 Isolates from Gynura procumbens

S. A. Choi; T. S. Park; J. S. Park; D. J. Min; J. S. Hong

doi:10.22698/jales.20190015

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2019 Vol.31, Issue 3 Preview Page Next Page

Research Article

Comparison of Genome Sequence and Biological Properties of Broad bean wilt virus 2 Isolates from Gynura procumbens 명월초에서 분리한 Broad bean wilt virus 2 분리주들의 염기서열 분석과 기주반응 비교

31 December 2019. pp. 121-127

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Abstract

Gynura procumbens (Myeongwolcho or longevity spinach) showing virus-like mosaic and yellowing symptoms was collected in Chuncheon. To identify the virus from G. procumbens, RT-PCR analysis was performed using several genus-specific primers sets. BBWV2-Gyp was identified from the amplified target product using Fabavirus genus-specific primers. BBWV2- Gyp had a similar host range pattern to BBWV2–SN that has already reported in G. procumbens. BBWV2-Gyp induced systemic mosaic and BBWV2-SN induced systemic necrotic spots on Phaseolus angularis. To investigate the sequence specificity, BBWV2-Gyp RNA2 was sequenced and compared with other BBWV2 isolates. Sequence homologies and phylogenetic analyses clearly indicated that BBWV2-Gyp had the closest relationship to BBWV2-SN. Also, BBWV2- Gyp had 98.5%, 98.8% and 98.5% homologies with movement protein (MP), large coat protein (LCP) and small coat protein (SCP) segments, respectively, of BBWV2-SN at amino acid level. Although two BBWV2 isolates from G. procumbens have high nucleotide sequence similarity (99%), they have distinct biological properties.

Keywords

Biological properties

Broad bean wilt virus 2

Gynura procumbens

Host range

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Information

Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
Publisher(Ko) :강원대학교 농업생명과학대학 농업생명과학연구원
Journal Title :Journal of Agricultural, Life and Environmental Sciences
Journal Title(Ko) :농업생명환경연구
Volume : 31
No :3
Pages :121-127
Received Date : 2019-12-04
Revised Date : 2019-12-18
Accepted Date : 2019-12-19
DOI :https://doi.org/10.22698/jales.20190015

Journal of Agricultural, Life and Environmental Sciences ISSN:2233-8322(Print) 2508-870X(Online) 농업생명환경연구

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