Prevalence of Campylobacter in Retail Raw Chickens and their Motility Phenotypes in South Korea

J. S. Kim; N. B. Y. Jeong; B. Y. Kim; S. Mun; E. S. Lim; O. K. Koo

doi:10.22698/jales.20200028

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2020 Vol.32, Issue 3 Preview Page Next Page

Research Article

Prevalence of Campylobacter in Retail Raw Chickens and their Motility Phenotypes in South Korea

31 December 2020. pp. 345-352

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Abstract

Campylobacter is a major food-borne pathogenic bacterium commonly found in poultry, including chickens. Therefore, it is necessary to study its prevalence among commercially available chickens. In this study, we investigated the prevalence of Campylobacter among commercially available raw whole chickens from three different sources, and chicken cuts from four different sources. Campylobacter was isolated from 23/30 (77%) whole chickens and 22/30 (73%) chicken cuts, with a 75% prevalence in total. Among the positive samples of whole chickens, C. jejuni and C. coli were isolated from 12 and 11 samples, respectively. In chicken cuts, the prevalence of C. coli was much lower at 4/30 samples (13%) than that of C. jejuni, which was isolated from 18/30 (60%) samples. The isolates were tested for motility as a virulence phenotype and compared to two human clinical strains, C. jejuni 11168 and 81-176. Compared to strain 11168, which was used as a control group with a motility index of 1.0, the mean motility index of the group of chicken isolates was 0.75. This was significantly lower than that of strain 11168 (P < 0.05), but similar to that of strain 81-176 (motility index of 0.74), a highly virulent strain. A high motility index (>1.0) was found only in C. jejuni. This study suggests that raw retail chickens continue to pose a significant threat to public health, and safe handling measures are necessary to prevent Campylobacter-associated food-borne illness among consumers.

Keywords

Campylobacter

Chicken

Motility

Prevalence

References

Bolton, D. J. (2015) Campylobacter virulence and survival factors. Food Microbiol 48:99-108. 10.1016/j.fm.2014.11.01725790997

Champion, O. L., Al-Jaberi, S., Stabler, R. A., Wren, B. W. (2008) Comparative genomics of Campylobacter jejuni. pp.63-71. In: Nachamkin et al. Campylobacter (3rd ed). ASM Press, Washington, DC, USA. 10.1128/9781555815554.ch4

European Food Safety Authority and European Centre for Disease Prevention and Control (EFSA and ECDC). (2018) The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2017. EFSA Journal 16:5500. 10.2903/j.efsa.2018.550032625785PMC7009540

Food Safety Nation. Foodborne illness statistics. (2020) Retrieved from https://www.foodsafetykorea.go.kr/portal/ healthyfoodlife/foodPoisoningStat.do?menu_no=3724&menu_grp=MENU_NEW02.

García-Sánchez, L., Melero, B., Diez, A. M., Jaime, I., Rovira, J. (2018) Characterization of Campylobacter species in Spanish retail from different fresh chicken products and their antimicrobial resistance. Food Microbiol 76:457-465. 10.1016/j.fm.2018.07.00430166174

Habib, I., De Zutter, L., Uyttendaele, M. (2013) Campylobacter species. pp.263-286. In: Doyle and Buchanan. Food microbiology: fundamentals and frontiers (4th ed). American Society for Microbiology, Washington, DC, USA. 10.1128/9781555818463.ch11

Havelaar, A. H., Kirk, M. D., Torgerson, P. R., Gibb, H. J., Hald, T., Lake, R. J., Praet, N., Bellinger, D. C., de Silva, N. R., Gargouri, N., Speybroeck, N., Cawthorne, A., Mathers, C., Stein, C., Angulo, F. J., Devleesschauwer, B. (2015) World Health Organization global estimates and regional comparisons of the burden of foodborne disease in 2010. PloS Med 12:e1001923. 10.1371/journal.pmed.100192326633896PMC4668832

Hodges, L. M., Carrillo, C. D., Upham, J. P., Borza, A., Eisebraun, M., Kenwell, R., Mutschall, S. K., Haldane, D., Schleihauf, E., Taboada, E. N. (2019) A strain comparison of Campylobacter isolated from retail poultry and human clinical cases in Atlantic Canada. PloS One 14:e0215928. 10.1371/journal.pone.021592831067242PMC6505856

Jung, G. H., Lim, E. S., Woo, M., Lee, J. Y., Kim, J., Paik, H. (2017) Inverse correlation between extracellular DNase activity and biofilm formation among chicken-derived Campylobacter strains. J Microbiol Biotechnol 27:1942-1951. 10.4014/jmb.1703.0305228870004

Kamar, R., Gohar, M., Jéhanno, I., Réjasse, A., Kallassy, M., Lereclus, D., Sanchis, V., Ramarao, N. (2013) Pathogenic potential of Bacillus cereus strains as revealed by phenotypic analysis. J Clin Microbiol 51:320-323. 10.1128/JCM.02848-1223135929PMC3536244

Kim, J., Park, H., Kim, J., Kim, J. H., Jung, J. I., Cho, S., Ryu, S., Jeon, B. (2019) Comparative analysis of aerotolerance, antibiotic resistance, and virulence gene prevalence in Campylobacter jejuni isolates from retail raw chicken and duck meat in South Korea. Microorganisms 7:433. 10.3390/microorganisms710043331658662PMC6843641

Korlath, J. A., Osterholm, M. T., Judy, L. A., Forfang, J. C., Robinson, R. A. (1985) A point-source outbreak of campylobacteriosis associated with consumption of raw milk. J Infect Dis 152:592-596. 10.1093/infdis/152.3.5924031557

Ma, H., Su, Y., Ma, L., Ma, L., Li, P., Du, X., Gölz, G., Wang, S., Lu, X. (2017) Prevalence and characterization of Campylobacter jejuni isolated from retail chicken in Tianjin, China. J Food Prot 80:1032-1040. 10.4315/0362-028X.JFP-16-56128504616

Marder, E. P., Cieslak, P. R., Cronquist, A. B., Dunn, J., Lathrop, S., Rabatsky-Ehr, T., Ryan, P., Smith, K., Tobin-D'Angelo, M., Vugia, D. J., Zansky, S., Holt, K. G., Wolpert, B. J., Lynch, M., Tauxe, R., Geissler, A. L. (2017) Incidence and trends of infections with pathogens transmitted commonly through food and the effect of increasing use of culture-independent diagnostic tests on surveillance - foodborne diseases active surveillance network, 10 U.S. sites, 2013-2016. MMWR-Morb Mortal Wkly Rep 66:397-403. 10.15585/mmwr.mm6615a128426643PMC5687182

Ministry of Food and Drug Safety. Korean Food Standards Codex. (2020) Retrieved from http://www.foodsafetykorea.go.kr/foodcode/01_01.jsp.

Moffatt, C. R. M., Fearnley, E., Bell, R., Wright, R., Gregory, J., Sloan-Gardner, T., Kirk, M., Stafford, R. (2020) Characteristics of Campylobacter gastroenteritis outbreaks in Australia, 2001 to 2016. Foodborne Pathog Dis 17:308-315. 10.1089/fpd.2019.273131738586

Park, S. F. (2002) The physiology of Campylobacter species and its relevance to their role as foodborne pathogens. Int J Food Microbiol 74:177-188. 10.1016/S0168-1605(01)00678-X

Rosner, B. M., Schielke, A., Didelot, X., Kops, F., Breidenbach, J., Willrich, N., Gölz, G., Alter, T., Stingl, K., Josenhans, C., Suerbaum, S., Stark, K. (2017) A combined case-control and molecular source attribution study of human Campylobacter infections in Germany, 2011-2014. Sci Rep 7:5139. 10.1038/s41598-017-05227-x28698561PMC5505968

Samad, A., Abbas, F., Ahmed, Z., Akbar, A., Naeem, M., Sadiq, M. B., Ali, I., Saima, Roomeela, Bugti, F. S., Achakzai, S. K. (2019) Prevalence, antimicrobial susceptibility, and virulence of Campylobacter jejuni isolated from chicken meat. J Food Safety 39:e12600. 10.1111/jfs.12600

Shyaka, A., Kusumoto, A., Chaisowwong, W., Okouchi, Y., Fukumoto, S., Yoshimura, A., Kawamoto, K. (2015) Virulence characterization of Campylobacter jejuni isolated from resident wild birds in Tokachi area, Japan. J Vet Med Sci 77:967-972. 10.1292/jvms.15-009025843040PMC4565820

Skirrow, M. B. (1977) Campylobacter enteritis: a "new" disease. Br Med J 2:9-11. 10.1136/bmj.2.6078.9871765PMC1631297

Stef, L., Cean, A., Vasile, A., Julean, C., Drinceanu, D., Corcionivoschi, N. (2013) Virulence characteristics of five new Campylobacter jejuni chicken isolates. Gut Pathog 5:41. 10.1186/1757-4749-5-4124330718PMC3866932

Thomas, M. K., Murray, R., Flockhart, L., Pintar, K., Pollari, F., Fazil, A., Nesbitt, A., Marshall, B. (2013) Estimates of the burden of foodborne illness in Canada for 30 specified pathogens and unspecified agents, circa 2006. Foodborne Pathog Dis 10:639-648. 10.1089/fpd.2012.138923659355PMC3696931

Tîrziu, E., Bărbălan, G., Morar, A., Herman, V., Cristina, R. T., Imre, K. (2020) Occurrence and antimicrobial susceptibility profile of Salmonella spp. in raw and ready-to-eat foods and Campylobacter spp. in retail raw chicken meat in Transylvania, Romania. Foodborne Pathog Dis 17:479-484. 10.1089/fpd.2019.273831990586PMC7415872

Van Cauteren, D., Le Strat, Y., Sommen, C., Bruyand, M., Tourdjman, M., Da Silva, N. J., Couturier, E., Fournet, N., de Valk, H., Desenclos, J. C. (2017) Estimated annual numbers of foodborne pathogen-associated illnesses, hospitalizations, and deaths, France, 2008-2013. Emerg Infect Dis 23:1486-1492. 10.3201/eid2309.17008128820137PMC5572882

Walker, L. J., Wallace, R. L., Smith, J. J., Graham, T., Saputra, T., Symes, S., Stylianopoulos, A., Polkinghorne, B. G., Kirk, M. D., Glass, K. (2019) Prevalence of Campylobacter coli and Campylobacter jejuni in retail chicken, beef, lamb, and pork products in three Australian states. J Food Prot 82:2126-2134. 10.4315/0362-028X.JFP-19-14631729918

Würfel, S. F. R., da Silva, W. P., de Oliveira, M. G., Kleinubing, N. R., Lopes, G. V., Gandra, E. A., Dellagostin, O. A. (2019) Genetic diversity of Campylobacter jejuni and Campylobacter coli isolated from poultry meat products sold on the retail market in Southern Brazil. Poultry Sci 98:932-939. 10.3382/ps/pey36530137619

Zhang, T., Luo, Q., Chen, Y., Li, T., Wen, G., Zhang, R., Luo, L., Lu, Q., Ai, D., Wang, H., Shao, H. (2016) Molecular epidemiology, virulence determinants and antimicrobial resistance of Campylobacter spreading in retail chicken meat in Central China. Gut Pathog 8:48. 10.1186/s13099-016-0132-227800028PMC5080698

Information

Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
Publisher(Ko) :강원대학교 농업생명과학대학 농업생명과학연구원
Journal Title :Journal of Agricultural, Life and Environmental Sciences
Journal Title(Ko) :농업생명환경연구
Volume : 32
No :3
Pages :345-352
Received Date : 2020-08-27
Revised Date : 2020-09-15
Accepted Date : 2020-09-21
DOI :https://doi.org/10.22698/jales.20200028

Journal of Agricultural, Life and Environmental Sciences ISSN:2233-8322(Print) 2508-870X(Online) 농업생명환경연구

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