Anti-Obesity Effect of Mixed Probiotics of Bifidobacterium animalis subsp. Lactis CP-9 and Lactobacillus rhamnosus bv-77 in High-Fat Diet-Induced Obese Mice

Mi-Ra Lee; Bohye Kim; Hee-Jun Kim; Eun-Joo Kum; Yeong-A Jung; Moonjin Ra; Bong-Hwan Chung; Yongjun Lee

doi:10.22698/jales.20210020

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2021 Vol.33, Issue 3S Preview Page Next Page

Research Article

Anti-Obesity Effect of Mixed Probiotics of Bifidobacterium animalis subsp. Lactis CP-9 and Lactobacillus rhamnosus bv-77 in High-Fat Diet-Induced Obese Mice 고지방식이로 유도한 비만 마우스에서 Bifidobacterium animalis subsp. lactis CP-9와 Lactobacillus rhamnosus bv-77 유산균 혼합제제의 항비만 효과

31 October 2021. pp. 194-204

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Abstract

The anti-obesity effect of a mixture of Bifidobacterium animalis subsp. lactis CP-9 and Lactobacillus rhamnosus bv-77 in an obese mouse model was studied. Obesity was induced in C57BL/6J mice by providing a 60 kcal% high fat diet (HFD) for 7 weeks. The mice were then administered probiotics for 5 weeks and divided into five groups as follows: (1) normal control group (10 kcal% fat diet), (2) HFD group (60 kcal% fat diet), (3) low dose-treated group (HFD + 5×10⁸ colony forming units (cfu)/mouse), (4) middle dose-treated group (HFD + 2.5×10⁹ cfu/mouse), and (5) high dose-treated group (HFD + 5×10⁹ cfu/mouse). Supplementation with the probiotic mixture did not affect plasma alanine aminotransferase and aspartate aminotransferase levels. Probiotic supplementation was decreased body weight gain, plasma lipid levels, and adipose tissue. In addition, mixed probiotics significantly altered the levels of plasma insulin, adiponectin, and leptin. Histological analysis of liver and epididymal adipose tissue also revealed reduced fat deposition and adipocyte size. These results suggest that the probiotic mixture of Bifidobacterium animalis subsp. lactis CP-9 and Lactobacillus rhamnosus bv-77 can alleviate diet-induced obesity and associated diseases by regulating fat accumulation, lipid metabolism, and the levels of leptin and adiponectin.

Keywords

Bifidobacterium animalis

High-fat diet

Lactobacillus rhamnosus

Obesity

Probiotics

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Information

Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
Publisher(Ko) :강원대학교 농업생명과학대학 농업생명과학연구원
Journal Title :Journal of Agricultural, Life and Environmental Sciences
Journal Title(Ko) :농업생명환경연구
Volume : 33
No :3
Pages :194-204
Received Date : 2021-09-28
Revised Date : 2021-10-14
Accepted Date : 2021-10-18
DOI :https://doi.org/10.22698/jales.20210020

Journal of Agricultural, Life and Environmental Sciences ISSN:2233-8322(Print) 2508-870X(Online) 농업생명환경연구

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