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2019 Vol.31, Issue 3 Preview Page

Research Article

Improving the Aglycon Isoflavone Content in Soybean Leaf Extracts by Lactic Acid Fermentation

젖산균 발효를 통한 콩잎 추출물의 비배당체 이소플라본 함량 증대

S. Y. Kwun1
,
J. A. Yoon1
,
E. H. Park2
,
M. D. Kim3*
권 세영1
,
윤 정아1
,
박 은희2
,
김 명동3*
1Ph.D. Course, Division of Food Biotechnology and Biosystems Engineering, Kangwon National University
2Ph.D. Course, Division of Food Biotechnology and Biosystems Engineering, Kangwon National University, CEO, Research and Development Institute, Metascreen Inc.
3Professor, Division of Food Biotechnology and Biosystems Engineering, Kangwon National University
1강원대학교 식품생명공학과 박사과정
2강원대학교 식품생명공학과 박사과정, (주)메타스크린 대표
3강원대학교 식품생명공학과 교수
* Corresponding Author
31 December 2019. pp. 160-170
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Abstract

A total of 112 lactic acid bacteria species were isolated from Kimchi prepared in Gangwon-do. Leuconostoc mesenteroides, a MSL129 strain with the highest β-glucosidase activity, was selected and identified by a sequence analysis of a 16S rRNA gene. The β-glucosidase activity of the Leu. mesenteroides MSL129 strain increased gradually with increases in reaction temperature. The optimum conditions for enzyme activity was at 45°C and pH 6.0. Bioconversion of soybean leaf extracts by the Leu. mesenteroides MSL129 strain yielded approximately three times higher levels of daidzein than the Leu. mesenteroides KCTC13388 strain. Leu. mesenteroides MSL129, an excellent bacterial selection for converting daidzin to daidzein in soybean extracts, was deposited as KCTC13578BP to the Korean Collection for Type Culture (KCTC).

Keywords
Bioconversion
β-Glucosidase, Isoflavone
Leuconostoc mesenteroides
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Information
  • Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
  • Publisher(Ko) :None
  • Journal Title :Journal of Agricultural, Life and Environmental Sciences
  • Volume : 31
  • No :3
  • Pages :160-170
  • Received Date : 2019-09-08
  • Revised Date : 2019-12-03
  • Accepted Date : 2019-12-05
  • DOI :https://doi.org/10.22698/jales.20190019
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