All Issue

2022 Vol.34, Issue 1 Preview Page

Research Article

Antioxidant and Cytotoxic Activities of Extracts from Aerial Parts and Roots of Ophiopogon planiscapus ‘Nigrescens’

흑맥문동 지상부와 뿌리 추출물의 산화방지 효과 및 세포독성 평가

Yeo Wan Son1
,
Smee Kang2
,
Ji Sun Lee3
,
Ju An Youn4
,
Mae Hee Chiang5*
손 여완1
,
강 스미2
,
이 지선3
,
윤 주안4
,
장 매희5*
1Ph.D Course, Dept. Horticulture, Biotechnology & Landscape Architecture, Seoul Women's University, Seoul 01797, Korea
2Researcher, Dept. Food Science & Technology, Seoul Women's University, Seoul 01797, Korea
3Ph.D Course, Dept. Horticulture, Biotechnology & Landscape Architecture, Seoul Women's University, Seoul 01797, Korea
4Master's Course, Dept. Horticulture, Biotechnology & Landscape Architecture, Seoul Women's University, Seoul 01797, Korea
5Professor, Dept. Horticulture, Biotechnology & Landscape Architecture, Seoul Women's University, Seoul 01797, Korea
1서울여자대학교 원예생명조경학과 박사과정
2서울여자대학교 식품공학과 연구원
3서울여자대학교 원예생명조경학과 박사과정
4서울여자대학교 원예생명조경학과 석사과정
5서울여자대학교 원예생명조경학과 교수
*Corresponding Author
31 March 2022. pp. 17-25
PDF XML Citation
Abstract

Ophiopogon planiscapus ‘Nigrescens’ is a perennial gardening plant, the bioactivities of which have rarely been studied. In the present study, the antioxidant activity and cytotoxic effect on intestinal cells of O. planiscapus were investigated. Methanol extracts were prepared from the aerial parts and roots of O. planiscapus and scavenging activities were investigated using the 1,1-diphenyl-2-picrylhydrazyl and 2,2΄-azobis-(3-ethylbenzothiazoline-6-sulfonic-acid) radicals, Fenton reaction-induced lipid peroxidation, and Fe2+ ion chelation assays, and cytotoxicity was evaluated against HCT 116 human colon cancer and INT-407 human normal intestinal cells. Total polyphenol and flavonoid levels were significantly higher in the root extracts of O. planiscapus than in the aerial parts. Scavenging activities against 1,1-diphenyl-2-picrylhydrazyl and 2,2΄-azobis-(3-ethylbenzothiazoline-6-sulfonic-acid) radicals of the root extracts (IC50 values of 0.32 and 0.16 mg/mL, respectively) were also more potent than those of the aerial part extracts (IC50 values of 1.19 and 0.41 mg/mL, respectively). However, nitrite scavenging activities of both extracts were not significantly different. Both extracts inhibited the Fenton reaction-induced lipid peroxidation through reducing thiobarbituric acid reactive substances and by chelating the Fe2+ ion. The extracts from the roots and aerial parts of O. planiscapus also exhibited concentration- dependent cytotoxicity against HCT 116 human colon cancer (IC50 of 51.6 and 98.4 µg/mL, respectively) and INT human normal intestinal (IC50 of 75.2 and 107.4 µg/mL, respectively) cells. Both extracts showed significantly higher potency to HCT 116 colon cancer cells. These results indicate that the extracts from roots and aerial parts of O. planiscapus contain biologically active compounds and can be used as a dietary source with health benefits.

Keywords
Antioxidant activity
Cytotoxicity
Lipid peroxidation
Radical scavenging activity
Ophiopogon planiscapus ‘Nigrescens’
References
1
Ambati, R. R., Ravi, S., Vallikannan, B., Gokare, A. R. (2006) Antioxidant activity of Botrycoccus braunii extract elucidated in vitro models. J Agr Food Chem 54:4593-4599. 10.1021/jf060799j16787003
2
Andjelković, M., Van Camp, J., De Meulenaer, B., Depaemelaere, G., Socaciu, C., Verloo, M., Verhe, R. (2006) Iron-chelation properties of phenolic acids bearing catechol and galloyl groups. Food Chemistry 98: 23-31. 10.1016/j.foodchem.2005.05.044
3
Blois, M. S. (1958) Antioxidant determination by the use of a stable free radical. Nature 26:1199-1203. 10.1038/1811199a0
4
Cowart, R. E., Singleton, F. L., Hind, J. S. (1993) A comparison of bathophenanthroline disulfonic acid and ferrozine as chelators of iron (II) in reduction reactions. Anal Biochem 211:151-155. 10.1006/abio.1993.12468323027
5
Friedman, M., Jürgens, H. S. (2000) Effect of pH on the stability of plant phenolic compounds. J Agr Food Chem 48:2101-2110. 10.1021/jf990489j10888506
6
Gray, J. I., Dugan, L.R. (1975) Inhibition of N-nitrosamine formation in model food systems. J Food Sci 40: 981-984. 10.1111/j.1365-2621.1975.tb02248.x
7
Hatier, J. H, Clearwater, M. J., Gould, K. S. (2013) The functional significance of black-pigmented leaves: photosynthesis, photoprotection and productivity in Ophiopogon planiscapus 'Nigrescens'. PLoS One 8:e67850. doi:10.1371/journal.pone.0067850 10.1371/journal.pone.006785023826347PMC3691134
8
Hong, J., Kim, H. J., Kim, J. Y. (2011) Factors affecting reactivity of various phenolic compounds with the Folin-Ciocalteu reagent. J Korean So Food Sci Nutr 40:205-213. 10.3746/jkfn.2011.40.2.205
9
Jara, P. J., Fulgencio, S. C. (2006) Effect of solvent and certain food constituents on different antioxidant capacity assays. Food Res Int 39:791-800. 10.1016/j.foodres.2006.02.003
10
Kim, H. J., Joo, N. R. (2008) Effect of different shading levels on the growth and leaf color changes of variegated Physocarpus 'Diabolo' and Ophipogon planiscapus 'Nigrescesn'. J Korean Env Res Reveg Tech 11:112-119.
11
Lee, K. W., Lee, H. J. (2006) The roles of polyphenols in cancer chemoprevention. Biofactors 26:105-121. 10.1002/biof.552026020216823097
12
Lee, N. H., Hong, J., Kim, J. Y., Chiang, M. H. (2009) Antioxidant properties and protective effects of Inula britannica var. chinensis regel on oxidative stress-induced neuronal cell damage. Korean J Food Sci Technol 41:87-92.
13
Lee, N. H., Jung, D. S., Hong, J. (2021) Antioxidant properties and protective effects of aerial parts from Cnidium officinale makino on oxidative stress-induced neuronal cell death. Prev Nutr Food Sci 26:200-208. 10.3746/pnf.2021.26.2.20034316485PMC8276706
14
Lloyd, R. V., Hanna, P. M., Mason, R. P. (1997) The origin of the hydroxyl radical oxygen in the Fenton reaction. Free Radical Biology and Medicine 22:885-888. 10.1016/S0891-5849(96)00432-7
15
Lu, Y., Dong, Y., Li, X. He, Q. (2016) The nitrite-scavenging properties of catechol, resorcinol, and hydroquinone: a comparative study on their nitration and nitrosation Reactions. J Food Sci 11:C2692-C2696. 10.1111/1750-3841.1353527741359
16
Oktay, M., Galvin, Ý., Küfrevioglu, Ö. Ý. (2003) Determination of in vitro antioxidant activity of fennel (Foeniculum vulgare) seed extracts. LWT - Food Sci Technol 36:263-271. 10.1016/S0023-6438(02)00226-8
17
Seo, S. J., Kim, M. W. (2010) Physiological activities of leaf and 5oot extracts from Liriope platyphylla. Korean J Food Preserv 17:123-130.
18
Udrea, A. M., Gradisteanu Pircalabioru, G., Boboc, A. A., Mares, C., Dinache, A., Mernea, M., Avram, S. (2021) Advanced bioinformatics tools in the pharmacokinetic profiles of natural and synthetic compounds with anti-diabetic activity. Biomolecules 11:1692. doi:10.3390/biom11111692 10.3390/biom1111169234827690PMC8615418
19
Villano, D., Fernandez-Pachon, M. S., Moya, M. L., Troncoso, A. M., Garcia-Parilla, M. C. (2007) Radical scavenging ability of phenolic compounds towards DPPH free radical. Talanta 71:230-235. 10.1016/j.talanta.2006.03.05019071293
Information
  • Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
  • Publisher(Ko) :강원대학교 농업생명과학대학 농업생명과학연구원
  • Journal Title :Journal of Agricultural, Life and Environmental Sciences
  • Journal Title(Ko) :농업생명환경연구
  • Volume : 34
  • No :1
  • Pages :17-25
  • Received Date : 2022-02-18
  • Revised Date : 2022-02-28
  • Accepted Date : 2022-03-04
  • An Erratum to this article was published on 31 December 2022.
    This article has been updated.
  • DOI :https://doi.org/10.22698/jales.20220003
Journal Informaiton Journal of Agricultural, Life and Environmental Sciences Journal of Agricultural, Life and Environmental Sciences
Online Submission & Review System
  • NRF
  • KOFST
  • KISTI Current Status
  • KISTI Cited-by
  • KCI 문헌 유사도 검사
  • orcid
  • open access
  • ccl
Journal Informaiton Journal Informaiton - close