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2021 Vol.33, Issue 3 Preview Page

Research Article

The Production and Characterization of Phytase by Germination in Barley

보리의 발아를 통한 Phytase 생산과 특징

Ha Guyn Sung1*
성 하균1*
1Professor, Department of Animal Science, Sangji University, Wonju 26339, Korea
1상지대학교 동물자원과학과 교수
*Corresponding Author
31 December 2021. pp. 329-338
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Abstract

As a study for producing abundantly activated phytase in the germinated barleys, which are grain feed materials, we investigated the changes and characteristics of phytase during germination in barley. When enzyme activity of phytase was measured after germination of corn, wheat, barley and rice under the same conditions as grains that can be used as feed materials, germinated barley showed the highest phytase activity of 2.91 ± 0.07 U/g compared to other grains (p < 0.05). The phytase activity of barley gradually increased with the times of germination, and decreased after peaking at 3.00 ± 0.32 U/g on the fourth day of germination. The temperature and pH of germinated barley phytase showed the highest peaks at 55°C and pH 5.0. Addition of 0.01% Tween 80 showed the best effects on the phytase activity of germinated barley. In all experiments, phytase activity had a tendency to decrease compared to the initial activity as the reaction time elapsed, but the treatment group of 0.01% Tween 80 maintained a similar level until 30 minutes after the reaction. Therefore, these results are considered to provide basic data for using germinated barley to improve the digestibility of feed-derived phytase and to prevent phosphate contamination of the environment through fecal excretion.

Keywords
Barley germination
Phosphate contamination
Phytase
Tween 80

본 연구는 phytase가 풍부히 활성화한 곡물사료 원료로서 발아 보리를 생산하기 위한 연구로 보리의 발아하는 동안 phytase의 효소 역가 변화 및 발아 보리 phytase의 효소 활성 특성에 대하여 연구하였다. 사료용 원료로 사용 가능한 곡류로서 옥수수, 밀, 보리 및 벼를 동일한 조건에서 발아 후 phytase의 효소 역가를 측정하였을 때 발아 보리의 phytase 역가가 2.91 ± 0.07 U/g으로 다른 곡류에 비하여 가장 높았다(p < 0.05). 발아 기간 동안 보리의 phytase 역가는 발아 시간이 경과함에 따라 점진적으로 증가하였고, 발아 4일차에 3.00 ± 0.32 U/g으로 최고 정점을 형성한 후에 감소하였다. 발아보리 phytase의 활성을 위한 온도 및 pH는 55°C 및 pH 5.0에서 최고 정점을 나타내었다. Tween 80이 발아보리의 phytase 활성에 미치는 영향은 0.01% 첨가하였을 때 가장 좋은 효과를 주었다. 그리고 모든 실험에서 반응시간이 경과함에 따라 초기 효소 역가에 비하여 낮아지는 경향을 보였으나, Tween 80을 0.01% 첨가한 처리구는 반응 후 30분까지 유사한 수준을 유지하였다. 따라서 이상과 같은 연구 결과는 사료 유래 phytate의 소화기 내 이용을 향상시키고 배설로 환경의 인산염 오염을 예방함에 있어 발아 보리의 동물 사료에 활용을 위한 기초 자료를 제공 할 것으로 사료된다.

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Information
  • Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
  • Publisher(Ko) :강원대학교 농업생명과학대학 농업생명과학연구원
  • Journal Title :Journal of Agricultural, Life and Environmental Sciences
  • Journal Title(Ko) :농업생명환경연구
  • Volume : 33
  • No :3
  • Pages :329-338
  • Received Date : 2021-10-05
  • Revised Date : 2021-11-10
  • Accepted Date : 2021-11-13
  • DOI :https://doi.org/10.22698/jales.20210032
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