Antagonistic Effects and Biochemical Properties of Bacteria Isolated from Soil

Hyun Seung Kim; Sang Woo Kim; Mahesh Adhikari; Youn Su Lee

doi:10.22698/jales.20210012

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2021 Vol.33, Issue 2 Preview Page Next Page

Research Article

Antagonistic Effects and Biochemical Properties of Bacteria Isolated from Soil 토양에서 분리한 세균의 길항효과 및 생화학적 특성

31 July 2021. pp. 125-135

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Abstract

This study was conducted to screen the antagonistic efficacy and biochemical characteristics of bacterial isolates from soil. In total, 39 different bacterial strains were isolated from the field rhizospheric soil collected from Bonghwa, Korea in 2017. Serial dilution technique was implied for isolation of bacterial isolates. Results of the in vitro antagonistic screening confirmed that the five bacterial strains have a potential for antagonism. The strains BH9 (Burkholderia sp.), BH29 (Lysinibacillus macroides), BH36 (Paenibacillus peoriae), BH37 (Bacillus subtilis), and BH38 (Burkholderia sp.) are bacterial isolates, antagonistic against different fungal phytopathogens (Alternaria solani, Colletotrichum gloeosporioides, Fusarium oxysporum, Rhizoctonia solani, Sclerotinia minor, and Sclerotinia sclerotiorum) that were obtained from the Korean Agriculture Culture Collection (KACC). The results suggest that BH37 possesses a high range of antagonism against all tested phytopathogenic fungi. However, all five bacterial isolates exhibited significant antagonistic potential; BH37 showed the highest mycelial inhibition of S. sclerotiorum by 95.3%. The five potential antagonistic bacterial isolates were biochemically characterized. Cellulase, chitinase, hydrogen cyanide, and protease production by these isolates were found to be positive in the biochemical characterization assay. Furthermore, highlighting the holistic mechanisms regarding the production of antifungal compounds and lipopeptides by these antagonistic bacterial isolates against phytopathogenic fungi would be worthwhile in the future.

Keywords

Antagonistic bacteria

Biochemical characterization

Phytopathogens

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Information

Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
Publisher(Ko) :강원대학교 농업생명과학대학 농업생명과학연구원
Journal Title :Journal of Agricultural, Life and Environmental Sciences
Journal Title(Ko) :농업생명환경연구
Volume : 33
No :2
Pages :125-135
Received Date : 2021-05-26
Revised Date : 2021-06-28
Accepted Date : 2021-07-05
DOI :https://doi.org/10.22698/jales.20210012

Journal of Agricultural, Life and Environmental SciencesISSN:2233-8322(Print) 2508-870X(Online)강원대학교 농업생명과학대학 농업생명과학연구원

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