Anti-obesity Effects of Treatment with Air-classified Brassica juncea L. Extract in High-Fat Diet-Induced Obese C57BL/6J Mice

Sung-Jin Park; Hyun-Duk Kim; Yoon-Hwan Lee

doi:10.22698/jales.20210038

All Issue

2021 Vol.33, Issue 3 Preview Page Next Page

Research Article

Anti-obesity Effects of Treatment with Air-classified Brassica juncea L. Extract in High-Fat Diet-Induced Obese C57BL/6J Mice 고지방식이로 유도된 C57BL/6J 마우스에서 공기분급 갓분말 추출물의 항비만 효과

31 December 2021. pp. 379-390

PDF XML

Abstract

Air-classified Brassica juncea L. extract (BJ) was investigated for its anti-obesity effects in C57BL/6J mice fed a high-fat diet (HFD). Mice were divided into two groups: one fed a normal diet (NFD), and the other, an HFD. Mice in the HFD-fed group were subsequently treated with BJ (50, 100, and 200 mg/kg/day) for 8 weeks. Inhibition of HFD-induced obesity by BJ treatment was evaluated by analyzing biochemical parameters and using real-time polymerase chain reaction assays. BJ supplementation significantly decreased the final body weight, adipose tissue size, and epididymal adipose tissue volume in comparison with those in the HFD-induced obese mice (p < 0.05). BJ treatment also decreased serum triglyceride, glucose, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol levels in obese mice. Furthermore, it attenuated HFD- induced obesity by downregulating C/EBPα, PPARγ, and SREBP-1c to suppress adipogenesis. Moreover, BJ treatment activated the AMPK pathway, increased ACC phosphorylation, and downstream CPT1 expression in HFD-induced obese mice. These results demonstrate that BJ has anti-obesity effects in vivo, indicating that it is an excellent candidate for developing anti-obesity functional foods.

Keywords

Air-classified Brassica juncea extract (BJ)

Anti-obesity

Body weight

C57BL/6J mice

High-fat diet

본 연구에서는 공기분급 갓분말 추출물이 고지방식이 섭취 비만 마우스의 비만 및 지질대사 개선에 미치는 영향을 검토하였다. C57BL/6 마우스의 체중은 100 mg/kg/day부터 유의적인 체중 감소를 보였으며, 100와 200 mg/kg/day 투여에 의한 농도 의존적인 유의적 차이는 나타나지 않았다. 갓분말 추출물 100과 200 mg/kg/day 농도에서 간과 내장지방 무게, 혈중 중성지방 함량, 총콜레스테롤함량, LDL-콜레스테롤함량, 지방세포 크기가 효과적으로 감소되었으며, 특히, 갓분말 추출물 200 mg/kg/day 농도에서 HFD군과 비교했을 때 부고환지방무게가 현저히 감소함을 나타내었다. 이와 같은 결과는 공기분급 갓분말 추출물이 transcription factor인 PPARγ, C/EBPα와 SREBP-1c의 발현을 유의적으로 감소하는 것에 기인하는 것으로 판단되며, 또한 지방조직에서 adiponectin을 증가시켜 AMPK pathway를 활성화하여 지방조직의 에너지 대사 장애를 개선하는 것으로 사료된다. 한편, 본 소재와 관련된 기능성분 및 작용기작에 대하여 추후 보다 구체적인 연구가 요구되며 이를 통해 기능성과 경쟁력을 갖춘 간편식, 영양 균형식으로의 개발이 기대된다.

References

Albu, J., Allison, D., Boozer, C. N., Heymsfield, S., Kissileff, H., Kretser, A., Krumhar, K., Leibei, R., Nonas, C., Pi-Sunyer, X., Vanltallie, T., Wedral, E. (1997) Obesity solutions: report of a meeting. Nutr Rev 55:150-156. 10.1111/j.1753-4887.1997.tb06469.x9212691

Back, S. N., Yang, J. L., Jin, H. H., Kim, Y. H. (2002) Effects of green tea consumption on serum lipid profiles. Korean J Nutr 35:854-862.

Cannon, B., Nedergaard, J. (2004) Brownadipose tissue: function and physiological significance. Physiological Rev 84:277-359. 10.1152/physrev.00015.200314715917

Choi, H., Eo, H., Park, K., Jin, M., Kim, S. H., Park, J. E., Kim, S. A. (2007) Water-soluble extract from Cucubita moschata shows anti-obesity effects by controlling lipid metabolism in a high fat diet-induced obesity mouse model. Biochemical and Biophysical Research Commun 359:419-425. 10.1016/j.bbrc.2007.05.10717548058

Czaja, A. J. (2014) Hepatic inflammation and progressive liver fibrosis in chronic liver disease. World J Gastroenterol 20:2515-2532. 10.3748/wjg.v20.i10.251524627588PMC3949261

Farrell, K. T. (1998) Spices, condiments and seasonings (2nd ed). pp.138-140. Springer Science & Business Media, Maryland, USA.

Freedman, D. S., Serdula, M. K., Perey, C. A., Ballew, C., Whitle L. (1997) Obesity levels of lipids and glucose, and smoking among Navajo adolescents. J Nutr 127:2120S-2127S. 10.1093/jn/127.10.2120S9339179

Grundy, S. M. (1998) Multifactorial causation of obesity: implications for prevention. Am J Clin Nutr 67:563S-572S. 10.1093/ajcn/67.3.563S9497171

Han, C. K., Kim, S. S., Choi, S. Y., Park, J. H., Lee, B. H. (2009) Effects of rice added with mulberry leaves and fruit on blood glucose, body fat and serum lipid levels in rats. J Korean Soc Food Sci Nutr 38:1336-1341. 10.3746/jkfn.2009.38.10.1336

Han, C. T., Lee, S. H., Lim, S. B., Kong, H. S., Kim, S. H., Lee, S. K. (2006) Studies on production of high fat diet induced obesity C57BL/6NCrj Bgi mice. Lab Anim Res 22:221-226.

Haslam, D. W., James, W. P. (2005) Obesity. Lancet 366:1197-1209. 10.1016/S0140-6736(05)67483-1

Jayasooriya, A. P., Sakono, M., Yukizaki, C., Kawano, M., Yamamoto, K., Fukuda, N. (2000) Effects of momordica charantia powder on serum glucose levels and various lipid parameters in rats fed with cholesterol-free and cholesterol-enriched diets. J Ethnopharmacol 72:331-336. 10.1016/S0378-8741(00)00259-2

Jung, E. Y., Lee, S. K. (2007) Anti-oxidant activities and regeneration effect in HaCaT cell line by Jeju Island aboriginal Eclonia cava. J Korean Soc Cosmetol 13:1071-1077.

Kajimura, S., Spiegelman, B. M., Seale, P. (2015) Brown and beige fat: physiological roles beyond heat generation. Cell Metabolism 22:546-559. 10.1016/j.cmet.2015.09.00726445512PMC4613812

Kim, H. J., Lee, Y. M., Kim, Y. H., Won, S. I., Choi, S. A., Choi, S. W. (2009) Inhibition of adipogenesis in 3T3-L1 adipocytes with Magnolia officinalis extracts. J Soc Cosmet Scientists Korea 35:117-123.

Kim, J. H., Park, J. H., Park, S. D., Choi, S. Y., Seong, J. H., Hoon, K. D. (2002) Preparation and antioxidant activity of health drink with extract powders from safflower (Carthamus tinctorius L.) seed. Korean J Food Sci 34:617-624.

King, D. J., Devaney, N. (1988) Clinical pharmacology of sibutramine hydrochloride (BTS 54524), a new antidepressant, in healthy volunteers. Br J Pharmacol 26:607-611. 10.1111/j.1365-2125.1988.tb05303.x3207566PMC1386640

Kopelman, P. G. (2000) Obesity as a medical problem. Nature 404:635-643. 10.1038/3500750810766250

Lee, J. M., Lee, S. H., Lee, J. H., Lee, E. (2006) Effects of Coix lachryma-jobi var. mayuen Stapf. pharmacopuncture on plasma lipid composition and glucose in rat fed high fat diet. Korean J Meridian & Acupoint 23:59-66.

Lee, M. R., Oh, D. S., Wee, A. J., Yun, B. S., Jang, S. A., Sung, C. K. (2014) Antiobesity effects of lentinus edodes on obese mice induced by high fat diet. J Korean Soc Food Sci Nutr 43:194-199. 10.3746/jkfn.2014.43.2.194

Lee, S. M., Rhee, S. H. (1997) Inhibitory effect of various cruciferous vegetables on the growth of human cancer cells. Korean J Life Sci 7:234-240.

Leung, W. Y., Thomas, G. N., Chan, J. C., Tomlinson, B. (2003) Weight management and current options in pharmacotherapy: orlistat and sibutramine. Clin Ther 25:58-80. 10.1016/S0149-2918(03)90009-9

Lowell, B. B., Spiegelman, B. M. (2000) Towards a molecular understanding of adaptive thermogenesis. Nature 404:652-660. 10.1038/3500752710766252

Musso, G., Gambino, R., Cassader, M. (2010) Obesity, diabetes, and gut microbiota: the hygiene hypothesis expanded? Diabetes Care 33:2277-2284. 10.2337/dc10-055620876708PMC2945175

Park, D. J., Ku, K. H., Kim, S. H. (1996) Characteristics and application of defatted soybean meal fractions obtained by microparticulation/air- classification. Korean J Food Sci Technol 28:497-505.

Park, J. H., Park, C., Han, M. H., Kim, B. W., Chung, Y. H., Kim, Y. H. (2011) Inhibition of adipocyte differentiation and adipogenesis by aged black garlic extracts in 3T3-L1 preadipocytes. J Life Sci 21:720-728. 10.5352/JLS.2011.21.5.720

Stranges, S., Dorn, J. M., Muti, P., Freudenheim, J. L., Farinaro, E., Russell, M., Nochajski, T. H., Trevisan, M. (2004) Body fat distribution, relative weight, and liver enzyme levels: a population-based study. Hepatology 39:754-763. 10.1002/hep.2014914999694

Visscher, T. L., Seidell, J. C. (2001) The public health impact of obesity. Annu Rev Public Health 22:355-375. 10.1146/annurev.publhealth.22.1.35511274526

Wada, K., Ikeda, Y. (1998) Longitudinal studies to determine the effect of body fat rate reduction on blood pressure. J Med Syst 22:19-25. 10.1023/A:10226982210399554106

Information

Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
Publisher(Ko) :강원대학교 농업생명과학대학 농업생명과학연구원
Journal Title :Journal of Agricultural, Life and Environmental Sciences
Journal Title(Ko) :농업생명환경연구
Volume : 33
No :3
Pages :379-390
Received Date : 2021-11-22
Revised Date : 2021-12-07
Accepted Date : 2021-12-09
DOI :https://doi.org/10.22698/jales.20210038

Journal of Agricultural, Life and Environmental Sciences ISSN:2233-8322(Print) 2508-870X(Online) 농업생명환경연구

All Issue