Journal of Agricultural, Life and Environmental Sciences ISSN:2233-8322(Print) 2508-870X(Online) 농업생명환경연구

Soil samples around charcoal kilns were analyzed in this study to confirm the possibility of isolating halophilic thermophiles from them. A marine agar medium was used for the isolation of halobacteria with cultivation at 37°C for several days. Five pure colonies were isolated using single colony isolation techniques. A 16S-based ID service was then performed to confirm the species of each strain identified. Results indicated that all the strains isolated were Bacillus species. Further study of these isolated strains was then undertaken to confirm whether they can be used industrially in the food, cosmetics, and other industries. This included examining whether these strains could be grown at high temperatures, as well as testing for enzyme productivity including amylase, lipase, and protease. Results demonstrated that five of these strains could grow at 55°C and possessed at least one enzyme activity. Findings also indicated that the isolated strains were halophilic thermophiles. These results have shown the possibility of the use of these strains in biological processes at high temperatures in the food and cosmetics industries. This study also contributes to securing domestic biological resources and the improvement of hydrolytic enzyme activity by using isolated strains of halophilic thermophiles.