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2018 Vol.30, Issue 1 Preview Page

Research Article

Antihypertensive Effect of Peptides from Sesame, Almond, and Pumpkin Seeds: In-silico and In-vivo Evaluation
R. Chelliah13
,
S. R. Ramakrishnan23
,
U. Antony3
,
S. H. Kim1
,
I. Khan1
,
C. N. Tango1
,
P. N. Kounkeu1
,
S. Wei1
,
M. S. Hussain1
,
E. B. M. Daliri1
,
R. Momna1
,
M. Y. Kwon1
,
E. H. Lee1
,
H. Y. Jo1
,
S. B. Hwang1
,
E. J. Park1
,
H. J. Kim1
,
D. H. Oh1*
,
,
,
,
,
,
,
,
,
,
,
,
,
,
,
,
,
1Department of Food Science and Biotechnology, College of Agriculture and Life Science, Kangwon National University
2School of Food Science and Biotechnology, Kyungpook National University
3Centre for Food Technology, Department of Biotechnology, Anna University
*교신저자. *Corresponding Author. 
March 2018. pp. 12-30
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Abstract

Plant proteins are important functional ingredients in many processed food products. In particular, globular proteins from various sources play an important role in many food products. In the current study, 11S globulin protein from white sesame seeds, amandin protein from almond seeds, and cucurbitin protein from pumpkin seeds were extracted by means of ammonium sulfate precipitation and purified by anion-exchange chromatography on a DEAE-Sephadex column (20 × 30 cm). Amandin protein of almond and cucurbitin protein of pumpkin seeds both belong to the 11S globulin family. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of protein samples treated with reducing agents revealed that the isolated 11S globulin from white sesame seeds was composed of an acidic (30－33 kDa) and basic (20－24 kDa) polypeptide, amandin from almond seeds was composed of an acidic (40－42 kDa) and basic (15－20 kDa) polypeptide, and cucurbitin from pumpkin seeds was composed of an acidic (35－42 kDa) and basic (20－25 kDa) polypeptide. The alpha amylase-inhibiting activities of the three proteins was determined. Cucurbitin of pumpkin seeds had a greater alpha amylase inhibitory effect (－86%) than 11S globulin of sesame (82.6%) and amandin protein of almond seeds (76%). The antihypertensive effect of the three proteins was evaluated by a chrioallantoin membrane assay in chick embryos, which revealed that cucurbitin protein showed higher vasodilatation activity than the other two proteins.

Keywords
Alpha amylase
Amandin
Antihypertensive
Cucurbitin
Globular proteins

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Information
  • Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
  • Publisher(Ko) :강원대학교 농업생명과학대학 농업생명과학연구원
  • Journal Title :Journal of Agricultural, Life and Environmental Sciences
  • Journal Title(Ko) :농업생명환경연구
  • Volume : 30
  • No :1
  • Pages :12-30
  • Received Date : 2017-11-22
  • Revised Date : 2018-02-09
  • Accepted Date : 2018-02-19
  • DOI :https://doi.org/10.12972/jales.20180002
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