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2020 Vol.32, Issue 2 Preview Page

Research Article

Validation of Analytical Method for Platycoside E and Platycodin D in Fermented Platycodon grandiflorum Root Extract

발효된 도라지 추출물의 지표성분(Platycoside E 및 Platycodin D) 분석법 검증

Y. E. Choi1
,
S. I. Choi1
,
X. Han1
,
X. Men1
,
G. W. Jang1
,
H. Y. Kwon1
,
B. W. Park2
,
J. J. Kim3
,
O. H. Lee4*
최 예은1
,
최 선일1
,
한 웅호1
,
문 효1
,
장 길웅1
,
권 희연1
,
박 병우2
,
김 정진3
,
이 옥환4*
1Graduate Student, Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 24341, Korea
2Manager, CombiMed Co. Ltd. Yanggu 24510, Korea
3CEO, CombiMed Co. Ltd. Yanggu 24510, Korea
4Professor, Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 24341, Korea
1강원대학교 바이오산업공학부 식품생명공학전공 대학원생
2(주)콤비메드 과장
3(주)콤비메드 대표
4강원대학교 바이오산업공학부 식품생명공학전공 교수
* Corresponding Author
31 July 2020. pp. 116-126
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Abstract

The aim of this study was to validate an analytical method for the determination of platycoside E and platycodin D in Platycodon grandiflorum root extract. Specificity, linearity, precision, accuracy, limit of detection (LOD), and limit of quantification (LOQ) of platycoside E and platycodin D were measured by high performance liquid chromatography. Correlation coefficients of the calibration curve for platycoside E and platycodin D were 0.9999 and 0.9997, respectively. The inter-day and intra-day precision values of platycoside E and platycodin D ranged from 0.58 to 3.59% and from 0.4 to 4.77%, respectively. The inter-day nd intra-day accuracies were 94.02 to 115.73% and 94.11 to 122.82%, respectively. The LOD for platycoside E and platycodin D was 0.22 µg/mL and 0.31 µg/mL, respectively. The results validated the analytical determination of platycoside E and platycodin D in Platycodon grandiflorum root extract.

Keywords
HPLC with Photodiode Array Detector
Method Validation
Platycodin D
Platycodon grandiflorum Root
Platycoside E
References
1
Byun, B. H. (2003) Antiobesity effects of Platycodon grandiflorum extract on body weight changes and serum lipid profiles of obese rats induced high fat diet. J Life Sci 13:896-902.
10.5352/JLS.2003.13.6.896
2
Choi, C. Y., Kim, J. Y., Kim, Y. S., Chung, Y. C., Hahm, K. S., Jeong, H. G. (2001) Augmentation of macrophage functions by an aqueous extract isolated from Platycodon grandiflorum. Cancer Lett 166:17-25.
10.1016/S0304-3835(01)00440-2
3
Choi, C. Y., Kim, J. Y., Kim, Y. S., Chung, Y. C., Seo, J. K., Jeong, H. G. (2001) Aqueous extract isolated from Platycodon grandiflorum elicits the release of nitric oxide and tumor necrosis factor-α from murine macrophages. Int Immunopharmacol. 1:1141-1151.
10.1016/S1567-5769(01)00047-9
4
Choi, S. H., Yoo, C. K., Hwang, J. H., Lee, G. B., Lee, Y. J., Lee, B. Y., Lee, O. H. (2017) Modification and validation of analytical method for oxypaeoniflorin and paeoniflorin in Moutan cortex radicis extract. J Korean Soc Food Sci Nutr 46:1091-1096.
5
Ha, Y. W., Na, Y. C., Seo, J. J., Kim, S. N., Linhardt, R. J., Kim, Y. S. (2006) Qualitative and quantitative determination of ten major saponins in Platycodi Radix by high performance liquid chromatography with evaporative light scattering detection and mass spectrometry. J Chromatogr A 1135:27-35.
10.1016/j.chroma.2006.09.01517007864PMC4142639
6
Jang, J. R., Hwang, S. Y., Lim, S. Y. (2011) Inhibitory effect of extracts of Platycodon grandiflorum (the ballon flower) on oxidation and nitric oxide production. Korean J Food Preserv 18:65-71.
10.11002/kjfp.2011.18.1.065
7
KFDA. (2015) Analytical method guideline about validation of drugs and etc. Korea Food & Drug Administration, Osong, Korea.
8
Kim, C. H., Jung, B. Y., Jung, S. K., Lee, C. H., Lee, H. S., Kim, B. H., Kim, S. K. (1986) Evaluation of antioxidant activity of Platycodon grandiflorum. Environ Toxicol 25:85-94.
9
Kim, M. S., Hur, Y. G., Kim, W. G., Park, B. W., Ahn, K. S., Kim, J. J., Bae, H. (2011) Inhibitory effect of Platycodon grandiflorum on TH1 and TH2 immune responses in a murine model of 2, 4-dinitrofluorobenzene-induced atopic dermatitis-like skin lesions. Ann Allergy Asthma Immunol 106:54-61.
10.1016/j.anai.2010.10.02021195946
10
Kim, H. K., Choi, J. S., Yoo, D. S., Choi, Y. H., Yon, G. H., Hong, K. S., Jeong, Y. C. (2007) HPLC analysis of saponins in Platycodi Radix. KOREAN J PHYSIOL PHA 38(2):192-196.
11
Kim, M. S., Kim, W. G., Chung, H. S., Park, B. W., Ahn, K. S., Kim, J. J., Bae, H. (2012) Improvement of atopic dermatitis-like skin lesions by Platycodon grandiflorum fermented by Lactobacillus plantarum in NC/Nga mice. BIOL PHARM BULL 35:1222-1229.
10.1248/bpb.b11050422863917
12
Kim, Y. H., Bae, D. B., Park, S. O., Lee, S. J., Cho, O. H., Lee, O. H. (2013) Method validation for the determination of eleutherosides and β-glucan in Acanthopanax koreanum. Korean J Food & Nutr 42:1419-1425.
10.3746/jkfn.2013.42.9.1419
13
Kim, Y. P., Lee, E. B., Kim, S. Y., Li, D., Ban, H. S., Lim, S. S., Ohuchi, K. (2001) Inhibition of prostaglandin E2 production by platycodin D isolated from the root of Platycodon grandiflorum. PLANTA MED 67:362-364.
10.1055/s-2001-1431711458457
14
KONISHI, T., TADA, A., SHOJI, J., KASAI, R., & TANAKA, O. (1978) The structures of platycodin A and C, monoacetylated saponins of the roots of Platycodon grandiflorum A. DC. CHEM PHARM BULL 26:668-670.
10.1248/cpb.26.668
15
Li, W., Zhang, M., Wang, Z., Wang, Y. P., Zheng, Y. N., Zhang, J. (2011) Simultaneous RP-HPLC determination of six platycosides: Application to an enzymatic preparation study. Chromatographia 74(11-12):777-782.
10.1007/s10337-011-2140-1
16
Li, W., Zhang, W., Xiang, L., Wang, Z., Zheng, Y. N., Wang, Y. P., Chen, L. (2010) Platycoside N: a new oleanane-type triterpenoid saponin from the roots of Platycodon grandiflorum. Molecules 15:8702-8708.
10.3390/molecules1512870221119565PMC6259122
17
Lim, K. H. (1971) A medicinal phytology (the details). p. 281. Dong Myoung Sa, Seoul, Korea.
18
Seo, E. K., Kim, K. S., Lee, T. K., Woo, D. Y., Kim, C. H., Lee, Y. C. (2000) Effect of dietary Platycodon grandiflorum on plasma glucose and lipid metabolism in KK−Aymice and streptozotocin-induced diabetic rats. Orient Pharm Exp Med 1:14-20.
19
Sung, N. J., Seo, J. K. (1998) Medical action of perennial Platycodon radix. 35-47. In proceeding Institute of Agricultural Resource Utilization Symposium for 50th anniversary GSNU. Institute of Agricultural Resource Utilization. Gyeongsang National University. Korea.
20
Tada, A., Kaneiwa, Y., Shoji, J., SHIBATA, S. (1975) Studies on the saponins of the root of Platycodon grandiflorum A. De Candolle. I. Isolation and the structure of platycodin-D. CHEM PHARM BULL 23:2965-2972.
10.1248/cpb.23.29651218442
21
Yan, Y. Z., Xue, J. C., Wu, J. R., Yoo, D. S., Lee, S. Y., Kim, Y. K., Park, S. U. (2012) Variation of triterpenoid saponin content in Platycodon grandiflorum (Jacq.) ADC. Asian J Chem 24:1268-1270.
22
Zhao, H. L., Cho, K. H., Ha, Y. W., Jeong, T. S., Lee, W. S., Kim, Y. S. (2006) Cholesterol-lowering effect of platycodin D in hypercholesterolemic ICR mice. Eur J Pharmacol 537:166-173.
10.1016/j.ejphar.2006.03.03216626693
Information
  • Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
  • Publisher(Ko) :None
  • Journal Title :Journal of Agricultural, Life and Environmental Sciences
  • Volume : 32
  • No :2
  • Pages :116-126
  • Received Date : 2020-06-25
  • Revised Date : 2020-07-21
  • Accepted Date : 2020-07-22
  • DOI :https://doi.org/10.22698/jales.20200012
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