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2020 Vol.32, Issue 2 Preview Page

Research Article

Validation of Analytical Method for Platycoside E and Platycodin D in Fermented Platycodon grandiflorum Root Extract

발효된 도라지 추출물의 지표성분(Platycoside E 및 Platycodin D) 분석법 검증

Y. E. Choi1
,
S. I. Choi1
,
X. Han1
,
X. Men1
,
G. W. Jang1
,
H. Y. Kwon1
,
B. W. Park2
,
J. J. Kim3
,
O. H. Lee4*
최 예은1
,
최 선일1
,
한 웅호1
,
문 효1
,
장 길웅1
,
권 희연1
,
박 병우2
,
김 정진3
,
이 옥환4*
1Graduate Student, Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 24341, Korea
2Manager, CombiMed Co. Ltd. Yanggu 24510, Korea
3CEO, CombiMed Co. Ltd. Yanggu 24510, Korea
4Professor, Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 24341, Korea
1강원대학교 바이오산업공학부 식품생명공학전공 대학원생
2(주)콤비메드 과장
3(주)콤비메드 대표
4강원대학교 바이오산업공학부 식품생명공학전공 교수
* Corresponding Author
31 July 2020. pp. 116-126
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Abstract

The aim of this study was to validate an analytical method for the determination of platycoside E and platycodin D in Platycodon grandiflorum root extract. Specificity, linearity, precision, accuracy, limit of detection (LOD), and limit of quantification (LOQ) of platycoside E and platycodin D were measured by high performance liquid chromatography. Correlation coefficients of the calibration curve for platycoside E and platycodin D were 0.9999 and 0.9997, respectively. The inter-day and intra-day precision values of platycoside E and platycodin D ranged from 0.58 to 3.59% and from 0.4 to 4.77%, respectively. The inter-day nd intra-day accuracies were 94.02 to 115.73% and 94.11 to 122.82%, respectively. The LOD for platycoside E and platycodin D was 0.22 µg/mL and 0.31 µg/mL, respectively. The results validated the analytical determination of platycoside E and platycodin D in Platycodon grandiflorum root extract.

Keywords
HPLC with Photodiode Array Detector
Method Validation
Platycodin D
Platycodon grandiflorum Root
Platycoside E
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Information
  • Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
  • Publisher(Ko) :강원대학교 농업생명과학대학 농업생명과학연구원
  • Journal Title :Journal of Agricultural, Life and Environmental Sciences
  • Journal Title(Ko) :농업생명환경연구
  • Volume : 32
  • No :2
  • Pages :116-126
  • Received Date : 2020-06-25
  • Revised Date : 2020-07-21
  • Accepted Date : 2020-07-22
  • DOI :https://doi.org/10.22698/jales.20200012
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