All Issue

2019 Vol.31, Issue 3 Preview Page

Research Article

Improving the Aglycon Isoflavone Content in Soybean Leaf Extracts by Lactic Acid Fermentation

젖산균 발효를 통한 콩잎 추출물의 비배당체 이소플라본 함량 증대

S. Y. Kwun1
,
J. A. Yoon1
,
E. H. Park2
,
M. D. Kim3*
권 세영1
,
윤 정아1
,
박 은희2
,
김 명동3*
1Ph.D. Course, Division of Food Biotechnology and Biosystems Engineering, Kangwon National University
2Ph.D. Course, Division of Food Biotechnology and Biosystems Engineering, Kangwon National University, CEO, Research and Development Institute, Metascreen Inc.
3Professor, Division of Food Biotechnology and Biosystems Engineering, Kangwon National University
1강원대학교 식품생명공학과 박사과정
2강원대학교 식품생명공학과 박사과정, (주)메타스크린 대표
3강원대학교 식품생명공학과 교수
* Corresponding Author
31 December 2019. pp. 160-170
PDF XML Citation
Abstract

A total of 112 lactic acid bacteria species were isolated from Kimchi prepared in Gangwon-do. Leuconostoc mesenteroides, a MSL129 strain with the highest β-glucosidase activity, was selected and identified by a sequence analysis of a 16S rRNA gene. The β-glucosidase activity of the Leu. mesenteroides MSL129 strain increased gradually with increases in reaction temperature. The optimum conditions for enzyme activity was at 45°C and pH 6.0. Bioconversion of soybean leaf extracts by the Leu. mesenteroides MSL129 strain yielded approximately three times higher levels of daidzein than the Leu. mesenteroides KCTC13388 strain. Leu. mesenteroides MSL129, an excellent bacterial selection for converting daidzin to daidzein in soybean extracts, was deposited as KCTC13578BP to the Korean Collection for Type Culture (KCTC).

Keywords
Bioconversion
β-Glucosidase, Isoflavone
Leuconostoc mesenteroides
References
1
Ahmed, A., Nasim, F. H., Batool, K., Bibi, A. (2017) Microbial β-glucosidase: sources, production and applications. Appl Environ Microbiol 5:31-46.
10.12691/jaem-5-2-2
2
Altschul, S. F., Gish, W., Miller, W., Myers, E. W., Lipman, D. J. (1990) Basic local alignment search tool. J Mol Biol 215:403-410.
10.1016/S0022-2836(05)80360-2
3
Axelson, M., Setchell, K. D. R. (1981) The excretion of lignans in rats-evidence for an intestinal bacterial source for this new group of compounds. FEBS Lett 123:337-342.
10.1016/0014-5793(81)80322-5
4
Chang, J. Y., Choi, Y. R., Chang, H. C. (2011) Change in the microbial profiles of commercial kimchi during fermentation. Korean J Food Preserv 18:786-794.
10.11002/kjfp.2011.18.5.786
5
Cho, G. Y., Han, S. I. (2015) Isolation and identification of β-glucosidase producing halophilic Roseivivax roseus. Korean J Microbiol 51:141-147.
10.7845/kjm.2015.5024
6
Diener, S., Zurbrugg, C., Tockner, K. (2009) Conversion of organic material by black soldier fly larvae: establishing optimal feeding rates. Waste Manag Res 27:603-610.
10.1177/0734242X0910383819502252
7
Duncan, D. B. (1955) Multiple range and multiple F tests. Biometrics 11:1-42.
10.2307/3001478
8
Hati, S., Vij, S., Singh, B. P., Mandal, S. (2013) β-Glucosidase activity and bioconversion of isoflavones during fermentation of soymilk. J Sci Food Agric 95:216-220.
10.1002/jsfa.674324838442
9
Ho, H. M., Chen, R., Huang, Y., Chen, Z. Y. (2002) Vascular effects of a soy leaves (Glycine max) extracts and kaempferol glycosides in isolated rat carotid arteries. Planta Med 68:487-491.
10.1055/s-2002-3254512094288
10
Hong, S. W., Lae, K. Y., Jung, B. M., Kim, W. S., Chung, K. S. (2009) Characterization of α-galactosidase and β-glucosidase by Weissella cibaria. Korean J Microbiol Biotechnol 37:204-212.
11
Jang, M. H., Kim, M. D. (2010) Exploration of β-glucosidase activity of lactic acid bacteria isolated from kimchi. Food Eng Prog 14:243-248.
12
Jang, M. H., Kim, M. D. (2011) β-1,4-Xylosidase activity of Leuconostoc lactic acid bacteria isolated from Kimchi. Korean J Food Sci Technol 43:169-175.
10.9721/KJFST.2011.43.2.169
13
Jang, M. S., Jung, K. E., Yun, J. U., Nam, K. H. (2016) Production and fermentation characteristics of seafood kimchi started with Leuconostoc mesenteroides SK-1 isolated from octopus baechu kimchi. Korean J Food Preserv 23: 1050-1057.
10.11002/kjfp.2016.23.7.1050
14
Jeong, S. Y., Park, C. S., Choi, N. S., Yang, H. J., Kim, C. Y., Yoon, B. D., Kang, D. O., Ryu, Y. W., Kim, M. S. (2011) Characteristics of bacteriocin produced by Lactococcus lactis ET45 isolated from kimchi. Kor J Microbiol 47:74-80.
15
Jung, T. D., Shin, G. H., Kim, J. M., Choi, S. I., Lee, J. H., Lee, S. J., Heo, I. Y., Park, S. J., Oh, S. K., Woo, K. S., Lim, J. K., Lee, O. H. (2016) Isoflavone, β-glucan content and antioxidant activity of defatted soybean powder by bioconversion with Lentinula edodes. J Food Hyg Saf 31:386-392.
10.13103/JFHS.2016.31.5.386
16
Kim, E. J., Shin, I. U., Kwun, S. Y., Park, E. H., Yi, J. H., Kim, M. D. (2019) Exploration of β-glucuronidase activity of lactic acid bacteria isolated from Kimchi. Microbiol Biotechnol Lett 47:434-440.
10.4014/mbl.1901.01002
17
Kim, I. B., Shin, S., Lim, B. L., Seong, G. S., Lee, Y. E. (2010) Bioconversion of soybean isoflavone by Lactobacillus plantarum and Bifidobacterium longum. Korean J Food Cookery Sci 26:214-219.
18
Kim, M. K., Lee, S. H., Hwang, K. (2011) Physicochemical properties of soybean leaf by cultivar and development of soybean curd prepared with soybean leaf powder. Korean J Food Cookery Sci 27:557-565.
10.9724/kfcs.2011.27.5.557
19
King, R. A., Broadbent, J. L., Head, R. J. (1996) Absorption and excretion of the soy isoflavone genistein in rats. J Nutr 126:176-182.
10.1093/jn/126.1.1768558299
20
Ku, S. M., You, H. J., Park, M. S., Ji, G. E. (2016) Whole-cell biocatalysis for producing ginsenoside Rd from Rb1 using Lactobacillus rhamnosus GG. J Microbiol Biotechnol 26:1206-1215.
10.4014/jmb.1601.0100227012233
21
Kumar, S., Stecher, G., Tamura, K. (2016) MEGA7: Molecular evolutionary genetics analysis version 7.0 for Bigger Datasets. Mol Biol Evol 33:1870-1874.
10.1093/molbev/msw05427004904
22
Lee, E. J., Kwon, J. E., Kim, S. J., Cha, M. S., Kim, I. H., Kang, S. C., Park, T. H. (2013) Isoflavones and biotransformed dihydrodaidzein production with in vitro cultured callus of korean wild arrowroot Pueraria lobata. J Plant Biotechnol 40:217-223.
10.5010/JPB.2013.40.4.217
23
Lee, H., Cha, S. S., Lee, M. Y., Chang, H. C., Lee, J. J. (2013) Acute toxicity of Leuconostoc citreum GR1 isolated from kimchi in mice. Korean J Food Preserv 20:121-126.
10.11002/kjfp.2013.20.1.121
24
Lee, K. H., Lee, J. H. (2011) Isolation of Leuconostoc and Weissella species inhibiting the growth of Lactobacillus sakei from kimchi. Koren J Microbiol Biotechnol 39:175-181.
25
Lee, K. I., Rhee, S. H., Han, J. K., Park, K. Y. (1995) Kinds and characteristics of traditional special kimchi in Pusan and Kyungnam province. J Korean Soc Food Nutr 24:734-743.
26
Lee, K. J., Jin, L. M., Kim, Y. S., Row, K. H. (2005) Recovery of aglycone of daidzein and genistein in soybeans. Korean Chem Eng Res 43:641-645.
27
Lee, K. W., Han, N. S., Kim, J. H. (2012) Purification and characterization of β-glucosidase from Weissella cibaria 37. J Microbiol Biotechnol 22:1705-1713.
10.4014/jmb.1206.0600723221534
28
Lee, M. H., Park, Y. H., Oh, H. S., Kwak, T. S. (2002) Isoflavone content in soybean and its processed products. Korean J Food Sci Technol 34:365-369.
29
Lee, S. H., Choi, D. J., Kim, J. G. (2003) Effect of salt concentration on soybean leaf kimchi fermentation. Korean J Food Preserv 10:512-516.
30
Lee, S. H., Choi, D. J., Kim, J. G. (2003) The effect of chitosan addition on soybean leaf kimchi fermentation. Korean J Food Preserv 10:517-521.
31
Oh, N. S., In, M. J. (2008) Production of a fermented soymilk using a new strain Leuconostoc mesenteroides KC51 isolated from Kimchi. J Korean Soc Appl Biol Chem 51:88-91.
32
Shim, K. S., Park, G. G., Park, Y. S. (2014) Bioconversion of puffed red ginseng extract using β-glucosidase producing lactic acid bacteria. Food Eng Prog 18:332-340.
10.13050/foodengprog.2014.18.4.332
33
Tamura, K., Nei, M. (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 10:512-526.
34
Verni, M., Wang, C., Montemurro, M., Angelis, M. D., Katina, K., Rizzello, C. G., Coda, R. (2017) Exploring the Microbiota of Faba Bean: Functional Characterization of Lactic Acid Bacteria. Front Microbiol 8:2461.
10.3389/fmicb.2017.0246129312174PMC5732949
35
Vitale, D. C., Piazza, C., Melilli, B., Drago, F., Salomone, S. (2013) Isoflavones: estrogenic activity, biological effect and bioavailability. Eur J Drug Metab Pharmacokinet 38:15-25.
10.1007/s13318-012-0112-y23161396
36
Wang, Y., Abdel-Rahman, M. A., Tashiro, Y., Xiao, Y., Zendo, T., Sakaic, K., Sonomoto, K. (2014) L-(+)-Lactic acid production by co-fermentation of cellobiose and xylose without carbon catabolite repression using Enterococcus mundtii QU 25. RSC Adv 4:22013-22021.
10.1039/C4RA02764G
37
Yi, E. J., Lee, J. M., Yi, T. H., Cho, S. C., Park, Y. J., Kook, M. C. (2012) Biotransformation of ginsenoside by Lactobacillus brevis THK-D57 isolated from kimchi Korean J Food Nutr 25:629-636.
10.9799/ksfan.2012.25.3.629
38
Yi, J. H., Park, H. S., Kwon, H. J., Kim, M. D., Kim, C. S. (2016) Effects of Leuconostoc mesenteroides AFY-3 on kimchi fermentation. J Agri Life Environ Sci 28:51-56.
39
Yoon, J. A., Kwun, S. Y., Park, E. H., Kim, M. D. (2019) Changes in isoflavone contents and physicochemical properties of soybean leaf extract by extraction conditions. Microbiol Biotechnol Lett 47:64-68.
10.4014/mbl.1812.12018
Information
  • Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
  • Publisher(Ko) :None
  • Journal Title :Journal of Agricultural, Life and Environmental Sciences
  • Journal Title(Ko) :농업생명환경연구
  • Volume : 31
  • No :3
  • Pages :160-170
  • Received Date : 2019-09-08
  • Revised Date : 2019-12-03
  • Accepted Date : 2019-12-05
  • DOI :https://doi.org/10.22698/jales.20190019
Journal Informaiton Journal of Agricultural, Life and Environmental Sciences Journal of Agricultural, Life and Environmental Sciences
Online Submission & Review System
  • NRF
  • KOFST
  • KISTI Current Status
  • KISTI Cited-by
  • KCI 문헌 유사도 검사_영문_불교학연구
  • orcid
  • open access
  • ccl
Journal Informaiton Journal Informaiton - close