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2022 Vol.34, Issue 1 Preview Page

Research Article

Antioxidant and Cytotoxic Activities of Extracts from Aerial Parts and Roots of Ophiopogon planiscapus ‘Nigrescens’

흑맥문동 지상부와 뿌리 추출물의 산화방지 효과 및 세포독성 평가

Yeo Wan Son1
,
Smee Kang2
,
Ji Sun Lee3
,
Ju An Youn4
,
Mae Hee Chiang5*
손 여완1
,
강 스미2
,
이 지선3
,
윤 주안4
,
장 매희5*
1Ph.D Course, Dept. Horticulture, Biotechnology & Landscape Architecture, Seoul Women's University, Seoul 01797, Korea
2Researcher, Dept. Food Science & Technology, Seoul Women's University, Seoul 01797, Korea
3Ph.D Course, Dept. Horticulture, Biotechnology & Landscape Architecture, Seoul Women's University, Seoul 01797, Korea
4Master's Course, Dept. Horticulture, Biotechnology & Landscape Architecture, Seoul Women's University, Seoul 01797, Korea
5Professor, Dept. Horticulture, Biotechnology & Landscape Architecture, Seoul Women's University, Seoul 01797, Korea
1서울여자대학교 원예생명조경학과 박사과정
2서울여자대학교 식품공학과 연구원
3서울여자대학교 원예생명조경학과 박사과정
4서울여자대학교 원예생명조경학과 석사과정
5서울여자대학교 원예생명조경학과 교수
*Corresponding Author
31 March 2022. pp. 17-25
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Abstract

Ophiopogon planiscapus ‘Nigrescens’ is a perennial gardening plant, the bioactivities of which have rarely been studied. In the present study, the antioxidant activity and cytotoxic effect on intestinal cells of O. planiscapus were investigated. Methanol extracts were prepared from the aerial parts and roots of O. planiscapus and scavenging activities were investigated using the 1,1-diphenyl-2-picrylhydrazyl and 2,2΄-azobis-(3-ethylbenzothiazoline-6-sulfonic-acid) radicals, Fenton reaction-induced lipid peroxidation, and Fe2+ ion chelation assays, and cytotoxicity was evaluated against HCT 116 human colon cancer and INT-407 human normal intestinal cells. Total polyphenol and flavonoid levels were significantly higher in the root extracts of O. planiscapus than in the aerial parts. Scavenging activities against 1,1-diphenyl-2-picrylhydrazyl and 2,2΄-azobis-(3-ethylbenzothiazoline-6-sulfonic-acid) radicals of the root extracts (IC50 values of 0.32 and 0.16 mg/mL, respectively) were also more potent than those of the aerial part extracts (IC50 values of 1.19 and 0.41 mg/mL, respectively). However, nitrite scavenging activities of both extracts were not significantly different. Both extracts inhibited the Fenton reaction-induced lipid peroxidation through reducing thiobarbituric acid reactive substances and by chelating the Fe2+ ion. The extracts from the roots and aerial parts of O. planiscapus also exhibited concentration- dependent cytotoxicity against HCT 116 human colon cancer (IC50 of 51.6 and 98.4 µg/mL, respectively) and INT human normal intestinal (IC50 of 75.2 and 107.4 µg/mL, respectively) cells. Both extracts showed significantly higher potency to HCT 116 colon cancer cells. These results indicate that the extracts from roots and aerial parts of O. planiscapus contain biologically active compounds and can be used as a dietary source with health benefits.

Keywords
Antioxidant activity
Cytotoxicity
Lipid peroxidation
Radical scavenging activity
Ophiopogon planiscapus ‘Nigrescens’
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Information
  • Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
  • Publisher(Ko) :None
  • Journal Title :Journal of Agricultural, Life and Environmental Sciences
  • Volume : 34
  • No :1
  • Pages :17-25
  • Received Date : 2022-02-18
  • Revised Date : 2022-02-28
  • Accepted Date : 2022-03-04
  • An Erratum to this article was published on 31 December 2022.
    This article has been updated.
  • DOI :https://doi.org/10.22698/jales.20220003
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