Genotoxicity Evaluation of Extracts from the Husks and Cobs of Purple Corn using an In vitro Chromosome Aberration Test in Chinese Hamster Lung Cells

Hee Yeon Kim; Ki Yeon Lee; Tae Hee Kim; Eun Jae Kim; Jong Yeol Park

doi:10.22698/jales.20210033

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2021 Vol.33, Issue 3 Preview Page Next Page

Research Article

Genotoxicity Evaluation of Extracts from the Husks and Cobs of Purple Corn using an In vitro Chromosome Aberration Test in Chinese Hamster Lung Cells 자색옥수수 포엽 및 속대추출물의 Chinese Hamster Lung 세포를 이용한 염색체 이상시험

31 December 2021. pp. 339-346

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Abstract

This study evaluated the toxicity of extracts from the husks and cobs of purple corn (HCPC) using an in vitro chromosomal aberration test in Chinese hamster lung (CHL) cells. Experimental groups were treated with –S9 mix (6 h), +S9 mix (6h), or –S9 mix (24 h). A dose range finding study was conducted by setting the concentrations of HCPC extracts to 156, 313, 625, 1,250, 2,500 and 5,000 µg/mL. Consequently, the –S9 mix (6h) RICC⁵⁵ value was 1677.76 µg/mL, no cytotoxicity was determined at +S9 mix (6h), and –S9 mix (24h) RICC⁵⁵ value was 582.91 µg/mL. Based on the results, concentration of experimental groups was determined by setting 1,000, 1,250, 1,500, and 1,750 µg/mL in –S9 mix (6 h); 1,250, 2,500, and 5,000 µg/mL in +S9 mix; and 100, 200, 400, 600, and 800 µg/mL in –S9 mix (24 h). The frequency of appearance of chromosomal dysplasia cells and numerical dysplasia cells was less than 5% each. In the positive controls, the frequency of appearance of structural dysplasia cells was more than 10%. Under the conditions of this study, extracts from the husks and cobs did not cause chromosome abnormalities in CHL cells.

Keywords

Chromosome aberration test

Cobs

Husk

Purple corn

자색 옥수수 포엽과 속대 추출물에 대한 염색체이상 유발여부를 평가하기 위하여 Chinese hamster 유래의 lung cell을 이용하여 직접법 (S9‑)과 대사활성법 (S9+)에서의 염색체이상시험을 실시하였다. 농도결정시험은 자색옥수수 포엽 및 속대 추출물의 농도를 156, 313, 625, 1,250, 2,500 및 5,000 µg/mL로 설정하며 실시하였다. 그 결과, ‑S9 mix RICC⁵⁵ 값은 1677.76 µg/mL, +S9 mix에서는 세포독성이 확인되지 않았으며 24 시간 처리에서 RICC⁵⁵ 값은 582.91 µg/mL이었다. 농도결정시험의 결과를 기초로 하여, ‑S9 mix에서는 1,000, 1,250, 1,500, 1,750, 2,000, 2,250 및 2,500 µg/mL, +S9 mix에서는 1,250, 2,500 및 5,000 µg/mL를 각각 설정하여 염색체이상시험(단시간처리법)을 실시하였고, 24 시간처리(연속처리법)에 대해서는 100, 200, 400, 600, 800 µg/mL 실시하였다. 표본관찰의 결과 ‑S9 mix와 +S9 mix, 연속처리법에서의 염색체구조이상세포 및 수적이상세포의 출현빈도는 각각 5% 미만이었고, 양성대조군의 경우 구조이상 세포의 출현빈도는 10% 이상이었다. 따라서 시험물질 시험물질은 본 시험조건 하에서 CHL 세포에 대한 염색체이상을 유발하지 않는 것(음성)으로 판단된다.

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Information

Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
Publisher(Ko) :강원대학교 농업생명과학대학 농업생명과학연구원
Journal Title :Journal of Agricultural, Life and Environmental Sciences
Journal Title(Ko) :농업생명환경연구
Volume : 33
No :3
Pages :339-346
Received Date : 2021-11-08
Revised Date : 2021-11-15
Accepted Date : 2021-11-16
DOI :https://doi.org/10.22698/jales.20210033

Journal of Agricultural, Life and Environmental Sciences ISSN:2233-8322(Print) 2508-870X(Online) 농업생명환경연구

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