Development and Validation of an Analytical Method for 2-Furoic Acid in Benincasa hispida Extracts (HR1901-W)

Sun-Il Choi, Xionggao Han, Xiao Men, Se-Jeong Lee, Mi-Hye Park, Ok-Hwan Lee, Jung-Mo Yang, Ye-Eun Choi, Ju-Hyun Cho

doi:10.22698/jales.20210030

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2021 Vol.33, Issue 3 Preview Page Next Page

Research Article

Development and Validation of an Analytical Method for 2-Furoic Acid in Benincasa hispida Extracts (HR1901-W) 동과 추출물(HR1901-W)의 지표성분 2-Furoic acid 분석법 개발 및 검증

31 December 2021. pp. 311-320

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Abstract

As interest and demand for functional foods increase, standardization becomes important to manage the quality of raw materials and products. Analytical methods for the standardization of bioactive compounds must be created in tandem with the development of functional foods. Therefore, the aim of this study was to validate an analytical method for the determination of 2-furoic acid in Benincasa hispida extract (HR1901-W). The 2-furoic acid analysis method was validated by evaluating the linearity, precision, accuracy, limit of detection (LOD), and limit of quantification (LOQ), using a high-performance liquid chromatography－photodiode array spectrum. Our results showed that the correlation coefficients of the calibration curve for 2-furoic acid was 0.9999. Furthermore, the LOD and LOQ for 2-furoic acid were 0.10 µg/mL and 0.32 µg/mL, respectively. The range of inter-day and intra-day precision values (RSDs, relative standard deviation) for 2-furoic acid were 0.13%－0.56% and 0.27%－0.33, respectively, and the inter-day and intra-day accuracy values (recovery) were 98.47%－102.74% and 98.77%－102.72%, respectively. In addition, the content of 2-furoic acid in HR1901-W was found to be 412.39 ± 0.91 µg/dry weight in grams. These results indicate that the analytical method used in this study should be appropriate for the quantitative analysis of 2-furoic acid in HR1901-W and considered for use as basic data for the standardization of HR1901-W.

Keywords

2-Furoic acid

Benincasa hispida

High-performance liquid chromatography

Method validation

Photodiode array spectrum

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Information

Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
Publisher(Ko) :강원대학교 농업생명과학대학 농업생명과학연구원
Journal Title :Journal of Agricultural, Life and Environmental Sciences
Journal Title(Ko) :농업생명환경연구
Volume : 33
No :3
Pages :311-320
Received Date :2021. 08. 06
Revised Date :2021. 10. 15
Accepted Date : 2021. 10. 18
DOI :https://doi.org/10.22698/jales.20210030

Journal of Agricultural, Life and Environmental Sciences ISSN:2233-8322(Print) 2508-870X(Online) 농업생명환경연구

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