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2021 Vol.33, Issue 3 Preview Page

Research Article

Development and Validation of an Analytical Method for 2-Furoic Acid in Benincasa hispida Extracts (HR1901-W)

동과 추출물(HR1901-W)의 지표성분 2-Furoic acid 분석법 개발 및 검증

Sun-Il Choi1
,
Xionggao Han1
,
Xiao Men1
,
Se-Jeong Lee1
,
Mi-Hye Park1
,
Ok-Hwan Lee2
,
Jung-Mo Yang3
,
Ye-Eun Choi3
,
Ju-Hyun Cho4*
최 선일1
,
한 웅호1
,
문 효1
,
이 세정1
,
박 미혜1
,
이 옥환2
,
양 정모3
,
최 예은3
,
조 주현4*
1Graduate Student, Department of Food Biotechnology and Environmental Science, Kangwon National University, Chuncheon 24341, Korea
2Professor, Department of Food Biotechnology and Environmental Science, Kangwon National University, Chuncheon 24341, Korea
3Researcher, Haram Central Research Institute, Cheongju 28160, Korea
4Chief Executive Officer, Haram Central Research Institute, Cheongju 28160, Korea
1강원대학교 농업생명과학대학 식품환경융합학과 식품생명공학 대학원생
2강원대학교 농업생명과학대학 식품환경융합학과 식품생명공학 교수
3하람 중앙연구소 연구원
4하람 중앙연구소 연구소장
*Corresponding Author
31 December 2021. pp. 311-320
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Abstract

As interest and demand for functional foods increase, standardization becomes important to manage the quality of raw materials and products. Analytical methods for the standardization of bioactive compounds must be created in tandem with the development of functional foods. Therefore, the aim of this study was to validate an analytical method for the determination of 2-furoic acid in Benincasa hispida extract (HR1901-W). The 2-furoic acid analysis method was validated by evaluating the linearity, precision, accuracy, limit of detection (LOD), and limit of quantification (LOQ), using a high-performance liquid chromatography－photodiode array spectrum. Our results showed that the correlation coefficients of the calibration curve for 2-furoic acid was 0.9999. Furthermore, the LOD and LOQ for 2-furoic acid were 0.10 µg/mL and 0.32 µg/mL, respectively. The range of inter-day and intra-day precision values (RSDs, relative standard deviation) for 2-furoic acid were 0.13%－0.56% and 0.27%－0.33, respectively, and the inter-day and intra-day accuracy values (recovery) were 98.47%－102.74% and 98.77%－102.72%, respectively. In addition, the content of 2-furoic acid in HR1901-W was found to be 412.39 ± 0.91 µg/dry weight in grams. These results indicate that the analytical method used in this study should be appropriate for the quantitative analysis of 2-furoic acid in HR1901-W and considered for use as basic data for the standardization of HR1901-W.

Keywords
2-Furoic acid
Benincasa hispida
High-performance liquid chromatography
Method validation
Photodiode array spectrum
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Information
  • Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
  • Publisher(Ko) :None
  • Journal Title :Journal of Agricultural, Life and Environmental Sciences
  • Volume : 33
  • No :3
  • Pages :311-320
  • Received Date : 2021-08-06
  • Revised Date : 2021-10-15
  • Accepted Date : 2021-10-18
  • DOI :https://doi.org/10.22698/jales.20210030
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