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2021 Vol.33, Issue 3 Preview Page

Research Article

Analysis of Free Amino Acids and Antioxidant Components by NaCl Treatment of Korean Native Apocynum lancifolium Russanov

NaCl처리에 따른 한국 자생 개정향풀의 유리아미노산 및 항산화 성분 분석

Jo-Hoon Kim1
,
Jae-Myun Lee1
,
Ye-Gun Park2
,
Mae-Hee Chiang3
,
Jung-Ae Baik4*
김 조훈1
,
이 재면1
,
박 예근2
,
장 매희3
,
백 정애4*
1Ph.D Course, Department of Horticulture, KongJu National University, Yae-San, Chungnam 32439, Korea
2Ph.D Course, Department of Food Science and Technology, KongJu National University, Yae-San, Chungnam 32439, Korea
3Professor, Department of Horticulture, Biotechnology & Landscape Architecture, Seoul Women's University, Seoul 01797, Korea
4Managing Director, Garden Education Center, Incheon 22001, Korea
1공주대학교 원예학과 박사과정
2공주대학교 식품공학과 박사과정
3서울여자대학교 원예생명조경학과 교수
4정원교육개발원 센터장
*Corresponding Author
31 December 2021. pp. 299-310
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Abstract

According to the free amino acid content including proline content after NaCl treatment, 21 free amino acids were analyzed. Glutamine content was the highest after 0.25% and 2.0% NaCl treatment and in the control, while arginine content was the highest after 0.5% and 1.0% NaCl treatment. The total free amino acid in Apocynum lancifolium followed the order 2.0% NaCl treatment > 0.5% NaCl treatment > 0.25% NaCl treatment > 1.0% NaCl treatment > control; in particular, the total free amino acid content after 2.0% NaCl treatment (7428.94 mg/kg) was about 3.4 times higher than that in the control (2218.94 mg/kg). The proline content after 2.0% NaCl treatment was 47.49 mg/kg, while it was the lowest in the control. Antioxidant content, such as flavonoid CE (catechin equivalent) and total polyphenol GAE (gallic acid equivalent), and antioxidant activity AAE (ascorbic acid equivalent) were the highest after 0.25% NaCl treatment (29034.17 mg CE/kg, 24607.68 mg GAE/kg, and 39231.67 mg AAE/kg, respectively).

Keywords
Antioxidant
Flavonoid
Free amino acid
Phenol
Proline

NaCl처리를 통한 개정향풀 전초에서 proline을 포함한 21개 유리아미노산의 성분이 분석되었으며 대조구와 0.25%, 2%농도의 NaCl처리 시 아미노산 성분 중 glutamine 성분이 21개 아미노산 종류 중 가장 많은 양으로 나타났고 0.5%, 1.0% 농도의 NaCl처리 시 아미노산 성분 중 arginine 성분이 가장 많은 양으로 나타났으며 전체 유리아미노산의 총량의 경우는 2.0% > 0.5%> 0.25% > 1.0% > 대조구 순의 결과를 보였고 특히 2.0% NaCl처리의 7428.94 mg/kg 값은 대조구 2218.94 mg/kg의 약 3.4배 이상 높았으며 또한 proline 함량도 47.49 mg/kg으로 나타났고 대조구의 경우 가장 낮은 값을 나타내었다. 항산화 물질인 플라보노이드 CE(catechin equivalent), 총폴리페놀 GAE(gallic acid equivalent), 그리고 항산화활성 AAE(ascorbic acid equivalent)는 모두 0.25% NaCl농도에서 가장 높게 나타났으며 그 값은 각각 29034.17 mg CE/kg, 24607.68 mg GAE/kg, 그리고 39231.67 mg AAE/kg 함량으로 분석되었다.

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Information
  • Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
  • Publisher(Ko) :None
  • Journal Title :Journal of Agricultural, Life and Environmental Sciences
  • Volume : 33
  • No :3
  • Pages :299-310
  • Received Date : 2021-09-08
  • Revised Date : 2021-09-29
  • Accepted Date : 2021-10-01
  • DOI :https://doi.org/10.22698/jales.20210029
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