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2024 Vol.36, Issue 2

Research Article

Protective Effects of Lactobacillus plantarum L-14 Supplementation Against High-fat Diet-induced Obesity and Metabolic Dysfunction in Mice

고지방식이 유도 비만 마우스에서 Lactobacillus plantarum L-14의 체지방 감소 효과

Mi-Ra Lee1
,
Bohye Kim2
,
Hee-Jun Kim2
,
Moonjin Ra3
,
Sooyoung Yang4
,
Sangkyu Park5
,
Jeongmin Seo6
,
Yongjun Lee7*
이 미라1
,
김 보혜2
,
김 희준2
,
라 문진3
,
양 수영4
,
박 상규5
,
서 정민6
,
이 용준7*
1Associated Researcher, Advanced Materials R&D Dept., Hongcheon Institute of Medicinal Herb, Hongcheon 25142, Korea
2Senior Researcher, Advanced Materials R&D Dept., Hongcheon Institute of Medicinal Herb, Hongcheon 25142, Korea
3Principal Investigator, Natural Products Chemistry R&D Dept., Hongcheon Institute of Medicinal Herb, Hongcheon 25142, Korea
4Senior Research Engineer, Strain Development Team, Bifido Co., Ltd., Gyeonggi, 12930, Korea
5Research Director, Biomedical Research Institute, NeoRegen Biotech Co., Ltd., Suwon, 16614, Korea
6Chief Executive Officer, NeoRegen Biotech Co., Ltd., Suwon, 16614, Korea
7Principal Investigator, Advanced Materials R&D Dept., Hongcheon Institute of Medicinal Herb, Hongcheon 25142, Korea
1(재)홍천메디칼허브연구소 신소재개발연구실 전임연구원
2(재)홍천메디칼허브연구소 신소재개발연구실 선임연구원
3(재)홍천메디칼허브연구소 천연물화학연구실장
4(주)비피도 균주개발팀 책임연구원
5(주)네오리젠바이오텍 연구소장
6(주)네오리젠바이오텍 대표
7(재)홍천메디칼허브연구소 신소재개발연구실장
*Corresponding Author
30 June 2024. pp. 65-76
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Abstract

This study aimed to evaluate the efficacy of Lactobacillus plantarum L-14 (L-14) supplementation in reducing body fat in high-fat diet (HFD)-induced obese mice. Mice were assigned to either a normal control diet or HFD, receiving varying doses of L-14 medium (ML, 2.5 × 108 cfu/mouse/day; MH, 5 × 108 cfu/mouse/day) and L-14 cell pellets (PL, 5 × 108 cfu/mouse/day; PH, 2 × 109 cfu/mouse/day) over a period of 7 weeks. The results showed significant reductions in body weight gain, food efficiency, and white adipose tissue weight in mice supplemented with L-14 compared to HFD-induced obese mice. Notably, the PH group showed substantial suppression of body weight gain by 17.44% and a 37.9% decrease in epididymal fat pads in HFD-fed mice. L-14 supplementation also normalized alanine aminotransferase and aspartate transaminase levels, alongside improvements in fasting hyperglycemia and dyslipidemia. Furthermore, L-14 treatment ameliorated insulin resistance, evidenced by restored insulin, leptin, and adiponectin levels. Additionally, L-14 mitigated hepatic steatosis by reducing fat droplet deposition and malondialdehyde levels, an oxidative stress biomarker, in HFD-fed mice. These results collectively indicate the protective effects of L-14 against HFD-induced obesity and associated metabolic diseases.

Keywords
High-fat diet
Insulin resistance
Lactobacillus plantarum L-14
Obesity
Probiotics
References
1

Abdelazez, A., Heba, A., Zhu, Z. T., Jia, F. F. (2018) Potential benefits of Lactobacillus plantarum as probiotic and its advantages in human health and industrial applications: A review. Adv Environ Biol 12:16-27.

10.22587/aeb.2018.12.1.4
2

Antoniades, C., Antonopoulos, A. S., Tousoulis, D., Stefanadis, C. (2009) Adiponectin: from obesity to cardiovascular disease. Obes Rev 10:269-279.

10.1111/j.1467-789X.2009.00571.x19389061
3

Bäckhed, F., Ding, H., Wang, T., Hooper, L. V., Koh, G. Y., Nagy, A., Semenkovich, C. F., Gordon, J. I. (2004) The gut microbiota as an environmental factor that regulates fat storage. Proc Natl Acad Sci 101:15718-15723.

10.1073/pnas.040707610115505215PMC524219
4

Behera, S. S., Ray, R. C., Zdolec, N. (2018) Lactobacillus plantarum with functional properties: An approach to increase safety and shelf-life of fermented foods. Biomed Res Int 2018:9361614.

10.1155/2018/936161429998137PMC5994577
5

Boqué, N., Campión, J., Paternain, L., García-Díaz, D. F., Galarraga, M., Portillo, M. P., Milagro, F. I., Ortiz de Solórzano, C., Martínez, J. A. (2009) Influence of dietary macronutrient composition on adiposity and cellularity of different fat depots in Wistar rats. J Physiol Biochem 65:387-396.

10.1007/BF0318593420358352
6

Busebee, B., Ghusn, W., Cifuentes, L., Acosta, A. (2023) Obesity: A review of pathophysiology and classification. Mayo Clin Proc 98:1842-1857.

10.1016/j.mayocp.2023.05.02637831039
7

Cai, H., Wen, Z., Zhao, L., Yu, D., Meng, K., Yang, P. (2022) Lactobacillus plantarum FRT4 alleviated obesity by modulating gut microbiota and liver metabolome in high-fat diet-induced obese. Food Nutr Res 66:7974.

10.29219/fnr.v66.797435721808PMC9180133
8

Chao, T., Kong, L., Shan, M., Lu, Z., Lu, Y. (2021) Protective and ameliorating effects of probiotics against diet-induced obesity: A review. Food Res Int 147:110490.

10.1016/j.foodres.2021.11049034399486
9

Choi, M. J., Yu, H., Kim, J. I., Seo, H., Kim, J. G., Kim, S. K., Lee, H. S., Cheon, H. G. (2023) Anti-obesity effects of Lactiplantibacillus plantarum SKO-001 in high-fat diet-induced obese mice. Eur J Nutr 62:1611-1622.

10.1007/s00394-023-03096-x36729332PMC10195764
10

Dixon, J. B., le Roux, C. W., Rubino, F., Zimmet, P. (2012) Bariatric surgery for type 2 diabetes. Lancet 379:2300-2311.

10.1016/S0140-6736(12)60401-222683132
11

Ellulu, M. S., Patimah, I., Khaza'ai, H., Rahmat, A., Abed, Y. (2017) Obesity and inflammation: the linking mechanism and the complications. Arch Med Sci 13:851-863.

10.5114/aoms.2016.5892828721154PMC5507106
12

Hao, Y., Li, J., Wang, J., Chen, Y. (2024) Mechanisms of health improvement by Lactiplantibacillus plantarum based on animal and human trials: A review. Fermentation 10:73.

10.3390/fermentation10020073
13

Jandhyala, S. M., Talukdar, R., Subramanyam, C., Vuyyuru, H., Sasikala, M., Reddy, D. N. (2015) Role of the normal gut microbiota. World J Gastroenterol 21:8787-8803.

10.3748/wjg.v21.i29.878726269668PMC4528021
14

Kaila, B., Raman, M. (2008) Obesity: A review of pathogenesis and management strategies. Can J Gastroenterol 22:61-68.

10.1155/2008/60903918209783PMC2659122
15

Klop, B., Elte, J. W., Cabezas, M. C. (2013) Dyslipidemia in obesity: Mechanisms and potential targets. Nutrients 5:1218-1240.

10.3390/nu504121823584084PMC3705344
16

Kwon, M., Lee, J., Park, S., Kwon, O. H., Seo, J., Roh, S. (2020) Exopolysaccharide isolated from Lactobacillus plantarum L-14 has anti-inflammatory effects via the Toll-like receptor 4 pathway in LPS-induced RAW264.7 cells. Int J Mol Sci 21:9283.

10.3390/ijms2123928333291425PMC7730553
17

Lee, J., Park, S., Oh, N., Park, J., Kwon, M., Seo, J., Roh, S. (2021) Oral intake of Lactobacillus plantarum L-14 extract alleviates TLR2- and AMPK-mediated obesity-associated disorders in high-fat-diet-induced obese C57BL/6J mice. Cell Prolif 54:e13039.

10.1111/cpr.1303933830560PMC8168423
18

Levin, B. E., Dunn-Meynell, A. A. (2002) Defense of body weight depends on dietary composition and palatability in rats with diet-induced obesity. Am J Physiol Regul Integr Comp Physiol 282:R46-R54.

10.1152/ajpregu.2002.282.1.R4611742822
19

Mehta, A., Marso, S. P., Neeland, I. J. (2017) Liraglutide for weight management: A critical review of the evidence. Obes Sci Pract 3:3-14.

10.1002/osp4.8428392927PMC5358074
20

Nataraj, B. H., Ali, S. A., Behare, P. V., Yadav, H. (2020) Postbiotics-parabiotics: the new horizons in microbial biotherapy and functional foods. Microb Cell Factories 19:168.

10.1186/s12934-020-01426-w32819443PMC7441679
21

Neyrinck, A. M., Rodriguez, J., Zhang, Z., Seethaler, B., Sánchez, C. R., Roumain, M., Hiel, S., Bindels, L., Cani, P. D., Paquot, N., Cnop, M., Nazare, J. A., Laville, M., Muccioli, G. G., Bischoff, S. C., Walter, J., Thissen, J. P., Delzenne, N. M. (2021) Prebiotic dietary fibre intervention improves fecal markers related to inflammation in obese patients: Results from the Food4Gut randomized placebo-controlled trial. Eur J Nutr 60:3159-3170.

10.1007/s00394-021-02484-533544206PMC8354918
22

Park, J., Kwon, M., Lee, J., Park, S., Seo, J., Roh, S. (2020) Anti-cancer effects of Lactobacillus plantarum L-14 cell-free extract on human malignant melanoma A375 cells. Molecules 25:3895.

10.3390/molecules2517389532859054PMC7503592
23

Prete, R., Garcia-Gonzalez, N., Di Mattia, C. D., Corsetti, A., Battista, N. (2020) Food-borne Lactiplantibacillus plantarum protect normal intestinal cells against inflammation by modulating reactive oxygen species and IL-23/IL-17 axis. Sci Rep 10:16340.

10.1038/s41598-020-73201-133004903PMC7529774
24

Purcell, M., Flores, Y. N., Zhang, Z. F., Denova-Gutiérrez, E., Salmeron, J. (2013) Prevalence and predictors of alanine aminotransferase elevation among normal weight, overweight and obese youth in Mexico. J Dig Dis 14:491-499.

10.1111/1751-2980.1207223678860
25

Settanni, L., Corsetti, A. (2008) Application of bacteriocins in vegetable food biopreservation. Int J Food Microbiol 121:123-138.

10.1016/j.ijfoodmicro.2007.09.00118022269
26

Shao, Y., Huo, D., Peng, Q., Pan, Y., Jiang, S., Liu, B., Zhang, J. (2017) Lactobacillus plantarum HNU082-derived improvements in the intestinal microbiome prevent the development of hyperlipidaemia. Food Funct 8:4508-4516.

10.1039/C7FO00902J29094115
27

Song, W., Song, C., Shan, Y., Lu, W., Zhang, J., Hu, P., Wu, X., Li, L., Guo, S. (2016) The anti-oxidative effects of three lactobacillus on high-fat diet induced obese mice. RSC Adv 6:65808-65815.

10.1039/C6RA06389F
28

Sorbara, M. T., Pamer, E. G. (2022) Microbiome-based therapeutics. Nat Rev Microbiol 20:365-380.

10.1038/s41579-021-00667-934992261
29

Tang, C., Kong, L., Shan, M., Lu, Z., Lu, Y. (2021) Protective and ameliorating effects of probiotics against diet-induced obesity: A review. Food Res Int 147:110490.

10.1016/j.foodres.2021.11049034399486
30

Tang, C., Lu, Z. (2019) Health promoting activities of probiotics. J Food Biochem 43:e12944.

10.1111/jfbc.1294431368544
31

Tian, L., Zhao, R., Xu, X., Zhou, Z., Xu, X., Luo, D., Zhou, Z., Liu, Y., Kushmaro, A., Marks, R., Dinnyés, A., Sun, Q. (2023) Modulatory effects of lactiplantibacillus plantarum on chronic metabolic disease. Food Sci Hum Wellness 12:959-974.

10.1016/j.fshw.2022.10.018
32

Torino, M. I., Front de Valdez, G., Mozzi, F. (2015) Biopolymers from lactic acid bacteria. Novel applications in foods and beverages. Front Microbiol 6:834.

10.3389/fmicb.2015.0083426441845PMC4566036
33

Turnbaugh, P. J., Hamady, M., Yatsunenko, T., Cantarel, B. L., Duncan, A., Ley, R. E., Sogin, M. L., Jones, W. J., Roe, B. A., Affourtit, J. P., Egholm, M., Henrissat, B., Heath, A. C., Knight, R., Gordon, J. I. (2009) A core gut microbiome in obese and lean twins. Nature 457:480-484.

10.1038/nature0754019043404PMC2677729
34

Yoo, S. R., Kim, Y. J., Park, D. Y., Jung, U. J., Jeon, S. M., Ahn, Y. T., Huh, C. S., McGregor, R., Choi, M. S. (2013) Probiotics L. Plantarum and L. Curvatus in combination alter hepatic lipid metabolism and suppress diet-induced obesity. Obesity 21:2571-2578.

10.1002/oby.2042823512789
35

Zhao, L., Shen, Y., Wang, Y., Wang, L., Zhang, L., Zhao, Z., Li, S. (2022) Lactobacillus plantarum S9 alleviates lipid profile, insulin resistance, and inflammation in high-fat diet-induced metabolic syndrome rats. Sci Rep 12:15490.

10.1038/s41598-022-19839-536109620PMC9478128
Information
  • Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
  • Publisher(Ko) :강원대학교 농업생명과학대학 농업생명과학연구원
  • Journal Title :Journal of Agricultural, Life and Environmental Sciences
  • Journal Title(Ko) :농업생명환경연구
  • Volume : 36
  • No :2
  • Pages :65-76
  • Received Date : 2024-03-22
  • Revised Date : 2024-04-08
  • Accepted Date : 2024-04-08
  • DOI :https://doi.org/10.22698/jales.20240006
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