Characteristics of Two Broad Bean Wilt Virus 2 Isolates from Perilla frutescens in Korea

Chaemin Shin; Youseop Shin; Dongjoo Min; Jisu Kim; Jin-Sung Hong

doi:10.22698/jales.20260023

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2026 Vol.38, Issue 3 Preview Page Next Page

Research Article

Characteristics of Two Broad Bean Wilt Virus 2 Isolates from Perilla frutescens in Korea

30 September 2026. pp. 338-344

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Abstract

Broad bean wilt virus 2 (BBWV2) is an important virus that infects Perilla frutescens; however, studies comparing the biological and molecular characteristics of regional isolates remain limited. In this study, perilla plants showing mosaic symptoms were collected from Chuncheon and Hwacheon, Korea in 2022. Reverse transcription polymerase chain reaction (RT-PCR) analysis detected BBWV2 in both samples, whereas perilla mosaic virus (PerMV), turnip mosaic virus (TuMV), and tomato spotted wilt virus (TSWV) were not detected. The two isolates were designated BBWV2-Ch and BBWV2-Hc. Sequence analysis showed that BBWV2-Ch shared the highest nucleotide sequence identity of 98.14% with the pepper isolate BBWV2-RP6 (GenBank accession no. JX183232.1), whereas BBWV2-Hc shared the highest identity of 97.10% with BBWV2-RP5 (JX183230.1). Biological characterization using nine host plants showed that both isolates induced mosaic symptoms in Nicotiana benthamiana and P. frutescens. However, distinct symptoms were observed in Chenopodium amaranticolor, indicating biological differences between the two isolates. Phylogenetic analysis based on the partial small coat protein amino acid sequence revealed that both isolates belonged to Group II-a, whereas the previously reported Korean perilla isolate, BBWV2-GS-PF, belonged to Group II-c. These results indicate that BBWV2 isolates infecting perilla in Korea comprise genetically diverse lineages. This study provides comparative biological and molecular characterization of two Korean perilla-derived BBWV2 isolates, offering a basis for further studies on the genetic diversity of BBWV2 in Korea.

Keywords

Biological characterization

Broad bean wilt virus

Perilla frutescens

Phylogenetic analysis

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Information

Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
Publisher(Ko) :None
Journal Title :Journal of Agricultural, Life and Environmental Sciences
Volume : 38
No :3
Pages :338-344
Received Date : 2026-08-05
Revised Date : 2026-08-24
Accepted Date : 2026-08-24
DOI :https://doi.org/10.22698/jales.20260023

Journal of Agricultural, Life and Environmental Sciences ISSN:2233-8322(Print) 2508-870X(Online)

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