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2025 Vol.37, Issue 4 Preview Page

Research Article

Characterization of the New Cucumber Mosaic Virus Isolated from Atractylodes macrocephala
Dongjoo Min1†
,
Gyeong Geun Min1†
,
Jisoo Park2
,
Chaemin Shin3
,
Jisu Kim3
,
Suk Hyun Park4
,
Ju-Yeon Yoon5
,
Rae-Dong Jeong6
,
Hangil Kim7
,
Jin-Sung Hong8*
1Ph.D. course, Interdisciplinary program in Smart Agriculture, Kangwon National University, Chuncheon 24341, Korea
2Researcher, Agriculture and Life Sciences Research Institute, Kangwon National University, Chuncheon 24341, Korea
3Undergraduate student, Department of Plant Medicine, Division of Bioresource Sciences, Kangwon National University, Chuncheon 24341, Korea
4Researcher, Gangwon State Agricultural Research and Extension Services, Gangwon State 24203, Korea
5Professor, Department of Plant Protection and Quarantine, Jeonbuk National University, Jeonju 54896, Korea
6Professor, Department of Applied Biology, Chonnam National University, Gwangju 61185, Korea
7Professor, Department of Forest Environment Protection, Kangwon National University, Chuncheon 24341, Korea
8Professor, Department of Plant Medicine, Division of Bioresource Sciences, Kangwon National University, Chuncheon 24341, Korea
*Corresponding Author
†These authors contributed equally to this work.
31 December 2025. pp. 417-424
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Abstract

Atractylodes macrocephala is a medicinal plant belonging to the genus Atractylodes, and it is widely cultivated in China and South Korea. To date, five viruses have been reported to infect A. macrocephala. In this study, A. macrocephala plants showing distortion symptoms were collected in Chuncheon, South Korea, followed by RT-PCR analysis using primer sets specific to these five viruses as well as primer sets targeting seven commonly occurring virus genera in South Korea. RT-PCR result revealed an amplicon of the expected size only with Cucumovirus-specific primers, and sequence analysis of the amplified product confirmed infection by cucumber mosaic virus (CMV). The newly identified isolate was designated CMV-Atm. CMV isolates from A. macrocephala have previously been reported in both China and South Korea. Therefore, the biological and molecular characteristics of CMV-Atm were compared with those of previously reported A. macrocephala-derived CMV isolates. CMV-Atm exhibited distinct pathogenicity and host responses compared with the previously identified isolates, although its phylogenetic analysis indicated high sequence similarity with them. Collectively, these findings suggest that CMV-Atm is genetically conserved among other A. macrocephala-infecting CMV isolates but displays unique symptom expression, implying subtle adaptations in its host-pathogen interactions.

Keywords
Atractylodes macrocephala
Coat protein
Cucumber mosaic virus
Host response
Phylogenetic analysis
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Information
  • Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
  • Publisher(Ko) :None
  • Journal Title :Journal of Agricultural, Life and Environmental Sciences
  • Volume : 37
  • No :4
  • Pages :417-424
  • Received Date : 2025-11-27
  • Revised Date : 2025-12-01
  • Accepted Date : 2025-12-01
  • DOI :https://doi.org/10.22698/jales.20250034
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