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2022 Vol.34, Issue 1 Preview Page

Research Article

Protective Mechanism of Cirsium japonicum var. maackii against Scopolamine-induced Cognitive Impairment

엉겅퀴의 스코폴라민 유도 인지능 손상에 대한 개선 효과 및 보호 기전 탐색

Min Jeong Kim1
,
Byeong Wook Noh2
,
Qi Qi Pang1
,
Sanghyun Lee3
,
Ji-Hyun Kim4
,
Eun Ju Cho5*
김 민정1
,
노 병욱2
,
팡 치치1
,
이 상현3
,
김 지현4
,
조 은주5*
1Ph.D Course, Department Food Science and Nutrition, Pusan National University, Busan 46241, Korea
2M.S Course, Department Food Science and Nutrition, Pusan National University, Busan 46241, Korea
3Professor, Department of Plant Science and Technology, Chung-Ang University, Anseong 17546, Korea
4Post-doctoral Fellow, Department Food Science and Nutrition, Pusan National University, Busan 46241, Korea
5Professor, Department Food Science and Nutrition, Pusan National University, Busan 46241, Korea
1부산대학교 식품영양학과 박사과정
2부산대학교 식품영양학과 석사과정
3중앙대학교 식물생명공학과 교수
4부산대학교 식품영양학과 박사후연구원
5부산대학교 식품영양학과 교수
*Corresponding Author
31 March 2022. pp. 73-87
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Abstract

Scopolamine induces cholinergic dysfunction and oxidative stress, which leads to cognitive impairment. In the present study, we investigated the mechanisms by which Cirsium japonicum var. maackii (CJM) improved cognitive impairment in scopolamine-injected mice. Mice were administered an ethyl acetate fraction of CJM (EACJM, 50 and 100 mg/kg) for 14 days, and were injected intraperitoneally with scopolamine (1.5 mg/kg) for 8 days. To determine the protective effect of EACJM against memory loss in mice, T-maze, novel object recognition (NOR), and Morris water maze (MWM) tests were conducted using a scopolamine-treated mouse model. The protective mechanisms of EACJM in the brain were studied using western blotting. Scopolamine- induced memory dysfunction in mice was confirmed using behavioral tests, including T-maze, NOR, and MWM. However, results showed that EACJM effectively alleviated memory impairment in scopolamine-treated mice. Brain weight of EACJM-treated mice increased compared to that of scopolamine-injected mice. EACJM also downregulated acetylcholinesterase and upregulated brain-derived neurotrophic factor and phosphorylated cAMP response element-binding protein, indicating that EACJM ameliorated scopolamine-induced cognitive impairment. In addition, EACJM downregulated the B-cell lymphoma-2-associated X protein/ B-cell lymphoma-2 ratio, indicating that EACJM blocked the apoptosis of neuronal cells. These results indicated that EACJM may be a useful therapeutic agent for preventing cognitive impairment in neurodegenerative diseases.

Keywords
Apoptosis
Cirsium japonicum var. maackii
Cognition
Mice
Scopolamine
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Information
  • Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
  • Publisher(Ko) :None
  • Journal Title :Journal of Agricultural, Life and Environmental Sciences
  • Volume : 34
  • No :1
  • Pages :73-87
  • Received Date : 2022-02-10
  • Revised Date : 2022-03-14
  • Accepted Date : 2022-03-15
  • DOI :https://doi.org/10.22698/jales.20220008
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