Bioassay based Korean Diamides Resistance Status and Management Strategy of Spodoptera exigua (Lepidoptera: Noctuidae)

Minyoung Choi; Minseop Noh; Dongjun Park; Hyeokchan Kwon; Juil Kim

doi:10.22698/jales.20260003

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2026 Vol.38, Issue 1 Preview Page Next Page

Research Article

Bioassay based Korean Diamides Resistance Status and Management Strategy of Spodoptera exigua (Lepidoptera: Noctuidae)

31 March 2026. pp. 36-42

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Abstract

Diamide insecticides have been widely used to control lepidopteran pests due to their high efficacy and selectivity, but their intensive and prolonged use has led to the development of resistance in Spodoptera exigua populations in Korea. This study investigated the current status of resistance to chlorantraniliprole in S. exigua, examined potential cross-resistance with flubendiamide, and assessed the inheritance pattern of resistance using hybrid strains. Field populations were collected from 19 locations between 2014 and 2022, and bioassays were conducted with 22 insecticides. Resistance ratios (RRs) were calculated based on the LC₅₀ of a susceptible strain, and F1 and F2 hybrids were generated through reciprocal crosses. The results showed high resistance levels to chlorantraniliprole and flubendiamide, particularly in populations from Yeoju, Jindo, and Gangneung. F2 hybrids exhibited an average mortality rate of only 35.6%, indicating stable inheritance of resistance. Moreover, no statistically significant differences were observed between reciprocal crosses, suggesting that resistance is inherited in an incomplete dominant manner and is likely located on an autosome. While molecular analyses were not performed in this study, previous research has indicated that overexpression of CYP9A40 may play a more critical role in resistance than known RyR mutations such as I4790M. These findings highlight the urgent need for insecticide resistance management (IRM) strategies, including the rotation of insecticides with different modes of action, the use of molecular diagnostics such as LAMP and qRT-PCR for field monitoring, and the integration of alternative compounds like meta-diamides and isoxazolines. A resistance-informed integrated pest management (IPM) approach will be essential to ensure sustainable and effective control of S. exigua.

Keywords

Diamide resistance

Integrated resistance management

Ryanodine receptor

Spodoptera exigua

References

Cho, S. L., Kyung, Y. J., Shin, S. E., Kang, W. J., Jung, D. H., Lee, S. J., Park, G. H., Kim, S. I., Cho, S. W., Kim, H. K., Koo, H. N., Kim, G. H. (2018) Susceptibility of field populations of Plutella xylostella and Spodoptera exigua to four diamide insecticides. Kor J Appl Entomol 57:43-50.

10.5656/KSAE.2018.02.0.009

Cordova, D., Benner, E. A., Sacher, M. D., Rauh, J. J., Sopa, J. S., Lahm, G. P., Selby, T. P., Stevenson, T. M., Flexner, M., Gutteridge, S., Rhoades, D. F., Wu, L., Smith, R. M., Tao, Y. (2006) Anthranilic diamides: a new class of insecticides with a novel mode of action, ryanodine receptor activation. Pest Biochem Phys 84:196-214.

10.1016/j.pestbp.2005.07.005

Han, C. H., Kim, J. I. (2024) Transcriptome profiling reveals novel insights into the regulation of calcium ion and detoxification genes driving chlorantraniliprole resistance in Spodoptera exigua. Heliyon 10:e40556.

10.1016/j.heliyon.2024.e4055639660196PMC11629198

Han, C. H., Rahman, M. M., Kim, J. I., Lueke, B., Nauen, R. (2024) Genome-wide analysis of detoxification genes conferring diamide insecticide resistance in Spodoptera exigua identifies CYP9A40. Chemosphere 367:143623.

10.1016/j.chemosphere.2024.143623

Han, C. H., Rahman, M. M., Shin, J. Y., Kim, J. H., Lee, S. H., Kwon, M., Timm, A. E., Ramasamy, S., Lee, Y. S., Kang, S. R., Park, S. H., Kim, J. I. (2023) Exaptation of I4790M mutation in ryanodine receptor of Spodoptera exigua (Lepidoptera: Noctuidae): Lessons from museum and field samples. Pest Biochem Phys 195:105579.

10.1016/j.pestbp.2023.105579

Kim, J. I., Khan, M., Lee, S. H., Nauen, R. (2025) Understanding and managing diamide insecticide resistance in lepidopteran pests: Insights into RyR mutations and metabolic mechanisms. Pest Biochem Phys 215:106629.

10.1016/j.pestbp.2025.106629

Lahm G.P., Cordova, D., Barry, J. D. (2009) New and selective ryanodine receptor activators for insect control. Bioorg Med Chem 17:4127-4133.

10.1016/j.bmc.2009.01.018

Liu, J. B., Li, F. Y., Dong, J. Y., Li, Y. X., Zhang, X. L., Wang, Y. H., Xiong, L. X., Li, Z. M. (2018) Anthranilic diamide derivatives as potential ryanodine receptor modulators: Synthesis, biological evaluation and structure-activity relationship. Bioorg Med Chem 26:3541-3550.

10.1016/j.bmc.2018.05.028

Nauen, R. (2006) Insecticide mode of action: return of the ryanodine receptor. PMS 62:690-692.

10.1002/ps.1254

Sattelle, D. B., Cordova, D., Cheek, T. R. (2008) Insect ryanodine receptors: molecular targets for novel pest control chemicals. Invert Neurosci 8:107-119.

10.1007/s10158-008-0076-4

Sparks, T. C., Nauen, R. (2015) IRAC: Mode of action classification and insecticide resistance management. Pest Biochem Phys 121:122-128.

10.1016/j.pestbp.2014.11.014

Tohnishi M., Nakao H., Furuya T., Seo A., Kodama H., Tsubata K., Fujioka Kodama, H., Hirooka, T., Nishimatsu T. (2005) Flubendiamide, a novel insecticide highly active against lepidopterous insect pests. J Pestic Sci 30:354-360.

10.1584/jpestics.30.354

Information

Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
Publisher(Ko) :None
Journal Title :Journal of Agricultural, Life and Environmental Sciences
Volume : 38
No :1
Pages :36-42
Received Date : 2025-11-25
Revised Date : 2025-12-27
Accepted Date : 2026-01-06
DOI :https://doi.org/10.22698/jales.20260003

Journal of Agricultural, Life and Environmental Sciences ISSN:2233-8322(Print) 2508-870X(Online)

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