Analysis of Enzyme Productivity of Halophilic Microorganisms Isolated from Freshwater to Search for Fermentative Strains with Possible Value for Functional Materials Development

Kee Yeol Kyong

doi:10.22698/jales.20210008

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2021 Vol.33, Issue 2 Preview Page Next Page

Research Article

Analysis of Enzyme Productivity of Halophilic Microorganisms Isolated from Freshwater to Search for Fermentative Strains with Possible Value for Functional Materials Development 기능성 소재 개발용 발효 균주 탐색을 위한 민물에서 분리한 호염성 미생물의 효소 생산능 분석

31 July 2021. pp. 83-92

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Abstract

This study characterized halophilic microorganisms isolated from freshwater in Korea. Samples were cultivated on marine agar at 37°C for several days. A total of 24 pure colonies obtained from single colony isolates were used for 16S rRNA-based phylogenetic analysis by an ID service located in EzBioCloud database. The obtained 16S rDNA sequences allocated the isolated strains were divided into 3 phyla, 11 families, 12 genera, and 17 species. To confirm whether the isolated strains were candidates for the fermentation of diverse food ingredients and development of functional cosmetic ingredients, amylase, lipase, and protease enzyme assays were performed individually; the tests revealed that 17 strains possessed at least one enzyme activity. In particular, 11 strains exhibited activities of 2 enzymes and 3 strains produced auxin. These strains might be useful in the food and cosmetic industries. Therefore, the study findings will contribute to securing domestic biological resources and improving hydrolytic enzyme activities using isolated strains.

Keywords

Enzyme

Fermentation

Freshwater

Halophilic

Microorganism

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Information

Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
Publisher(Ko) :강원대학교 농업생명과학대학 농업생명과학연구원
Journal Title :Journal of Agricultural, Life and Environmental Sciences
Journal Title(Ko) :농업생명환경연구
Volume : 33
No :2
Pages :83-92
Received Date : 2021-04-26
Revised Date : 2021-05-20
Accepted Date : 2021-05-21
DOI :https://doi.org/10.22698/jales.20210008

Journal of Agricultural, Life and Environmental Sciences ISSN:2233-8322(Print) 2508-870X(Online) 농업생명환경연구

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