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2026 Vol.38, Issue 2 Preview Page
Evaluation of Antioxidant Activity in Euphorbia humifusa Extracts
Na-Young Kim1
,
Jin-Hyuk Choi2
,
Sung-Jin Park3*
1Professor, Department of Hotel Tourism Culinary Arts, Songho University, Hoengseong 25242, Korea
2Ph.D. Student, Department of Culinary and Food Service Management, Sejong University, Seoul 05006, Korea
3Professor, Department of Liberal Studies, Hallym Sacred Heart University, Chuncheon 24210, Korea
*Corresponding Author
30 June 2026. pp. 195-208
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Abstract

Euphorbia humifusa is a widely distributed medicinal herb rich in phenolic compounds; however, comprehensive evaluations of its phenolic profile and antioxidant potential using methanolic extracts remain limited. Dried aerial parts were extracted with 80% methanol (yield 20.32%). The methanolic extract contained high levels of gallic acid (11.29 mg/g), TPC (164.21 ± 0.45 mg GAE/g), and TFC (53.62 ± 0.18 mg RE/g). The extract exhibited dose-dependent antioxidant activity, achieving 81.25% DPPH scavenging at 400 µg/mL, strong ABTS activity comparable to standard antioxidants at higher concentrations, and a FRAP value of 584.32 ± 1.62 µmol Fe(II)/g. Pearson correlation analysis revealed very strong positive relationships between TPC and antioxidant assays (e.g., DPPH R = 0.998, ABTS R = 0.997). The antioxidant capacity of E. humifusa methanolic extract is substantial and is largely attributable to its high phenolic content -particularly gallic acid - supporting its potential as a natural antioxidant source for food, cosmetic, or nutraceutical applications. Further work is recommended to isolate active constituents, assess in vivo efficacy and safety, and optimize extraction and standardization protocols.

Keywords
Antioxidative activity
Euphorbia humifusa
Free radical scavenging activity
Nutritional components
Polyphenols
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Information
  • Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
  • Publisher(Ko) :None
  • Journal Title :Journal of Agricultural, Life and Environmental Sciences
  • Volume : 38
  • No :2
  • Pages :195-208
  • Received Date : 2026-05-11
  • Revised Date : 2026-05-21
  • Accepted Date : 2026-05-26
  • DOI :https://doi.org/10.22698/jales.20260014
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