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2017 Vol.29, Issue 3 Preview Page
Evaluation of Anti-obesity Effect of Roasted Inonotus obliquus in 3T3-L1 Cells

분화된 3T3-L1 세포에서 로스팅 차가버섯의 항비만 효능 평가

N. E. Kim1
,
M. O. Lee1
,
M. H. Jang1
,
B. H. Chung1*
김 나은1
,
이 미옥1
,
장 미희1
,
정 병희1*
1Research and Development Institute, Haesong KNS
1(주)해송KNS 기업부설연구소
*Corresponding Author. 
December 2017. pp. 193-201
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Abstract

The anti-obesity effects of extracts of Inonotus obliquus and roasted I. obliquus were investigated through the measurement of the differentiation of 3T3-L1 pre-adipocytes to adipocytes. Cytotoxic activity was determined by MTT assays on 3T3-L1 pre-adipocyte. Adipocyte differentiation was assessed through the evaluation of lipid accumulation (by using Oil Red O staining) and the expression of adipogenic proteins (by using western blotting). Compared with the control, the treatment of differentiating adipocytes with extracts of I. obliquus , and roasted I. obliquus significantly inhibited differentiation. In the presence of an adipogenic cocktail (isobutyl- methylxanthine, dexamethasone, and insulin), I. obliquus and roasted I. obliquus extracts significantly decreased the expression of adipogenic proteins, peroxisome proliferator-activated receptor-γ (PPARγ), and CCAAT/enhancer binding protein alpha (C/EBP-α), relative to the expression level of N-acetyl-L-cystein (NAC, negative control). These results indicated that the extracts of I. obliquus and roasted I. obliquus were potential candidates for the prevention obesity.

Keywords
Anti-obesity
Inonotus obliquus
Lipid accumulation
Roasting

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Information
  • Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
  • Publisher(Ko) :강원대학교 농업생명과학대학 농업생명과학연구원
  • Journal Title :Journal of Agricultural, Life and Environmental Sciences
  • Journal Title(Ko) :농업생명환경연구
  • Volume : 29
  • No :3
  • Pages :193-201
  • Received Date : 2017-11-30
  • Revised Date : 2017-12-18
  • Accepted Date : 2017-12-18
  • DOI :https://doi.org/10.12972/jales.20170018
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