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2017 Vol.29, Issue 2 Preview Page
Transcriptomic expression profiling of seedlings of wheat (Triticum aestivum L.) under ABA treatment

유묘기 밀(Triticum aestivum L.)에서의 ABA 처리 시 전사체 발현 분석

Y. J. Lee1
,
D. Y. Kim1
,
Y. W. Seo1*
이 용진1
,
김 대연1
,
서 용원1*
1Department of Biotechnology, Korea University
1고려대학교 생명공학과
*Corresponding Author. 
July 2017. pp. 78-86
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Abstract

One of the major phytohormones, ABA, is known to play an important role in seed dormancy, germination and response to various stresses. High-throughput sequencing technology was applied to ABA treated wheat seedlings to identify differentially expressed genes (DEGs), and we obtained 65,561,602 raw reads resulting from Illumina sequencing. After quality control and mapping to the draft reference genes of wheat, we identified 2,238 DEGs, and gene ontology (GO) analysis of the DEGs was further conducted. Based on these results, several candidate genes involved in ABA pathways were identified (aldehyde oxidase, β glucosidase and cytochrome P450). The combination of expression pattern analysis of high-throughput sequencing and GO analysis in this study will provide powerful clues and information for investigating the transcriptional changes to understand the transcriptional responses to ABA in wheat.

Keywords
ABA
DEGs
Wheat

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Information
  • Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
  • Publisher(Ko) :None
  • Journal Title :Journal of Agricultural, Life and Environmental Sciences
  • Journal Title(Ko) :농업생명환경연구
  • Volume : 29
  • No :2
  • Pages :78-86
  • Received Date : 2017-07-05
  • Revised Date : 2017-07-14
  • Accepted Date : 2017-07-14
  • DOI :https://doi.org/10.12972/jales.20170008
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