All Issue

2025 Vol.37, Issue 1 Preview Page

Research Article

Artificial White LED Light Influences Plant Growth and Centelloside Accumulation of Centella asiatica Grown in a Hydroponic System
Md Rayhan Ahmed Shawon1
,
Md Obyedul Kalam Azad2
,
Byeong Ryeol Ryu3
,
Jong Kuk Na4
,
Ki Young Choi14*
1Postdoctoral Researcher, Environmental Research Institute, Kangwon National University, Chuncheon 24341, Korea
2Senior Scientist, Department of Agriculture, Veterinary and Rangeland Sciences, University of Nevada, NV 89030, USA
3Graduate Student, Department of Bio-Health Convergence, Kangwon National University, Chuncheon 24341, Korea
4Professor, Department of Smart Farm and Agricultural Industry, Kangwon National University, Chuncheon 24341, Korea
*Corresponding Author
31 March 2025. pp. 16-25
PDF XML Citation
Abstract

Centella asiatica is a perennial medicinal herb, a rich source of triterpenoids including centelloside (asiaticoside, madecassoside, asiatic acid, and madecassic acid). Light intensity is an important factor that enhances plant growth and the accumulation of these triterpenoids in C. asiatica. The objective of this study was to investigate the optimal light intensity that enhances the growth of C. asiatica and centelloside accumulation. The plants were hydroponically grown using the nutrient film technique for four weeks under three different light intensities of white, fluorescent light at 50 (L50), 150 (L150), and 300 (L300) photosynthetic photon flux density (PPFD), maintaining a 16/8 h light/dark cycle with relative humidity at 65 ± 5%. The results revealed that morphological features of C. asiatica such as petiole length, number of leaves, leaf area, number of runners, shoot fresh weight, and shoot dry weight were significantly higher in plants treated with L150. However, the highest content of asiaticoside, madecassoside, and asiatic acid was observed in L300-treated plants. Additionally, principal component analysis (PCA) indicated that the number of leaves, leaf area, shoot fresh weight, and SPAD value are positively correlated. These results suggest that although the L300 treatment hinders the morphological development of C. asiatica, it is preferable for centelloside biosynthesis.

Keywords
Light intensities
Number of leaves
Nutrient film technique
Principal component analysis
Triterpenoids
References
1

Bian, Z., Jiang, N., Grundy, S., Lu, C. (2017) Uncovering LED light effects on plant growth: new angles and perspectives-LED light for improving plant growth, nutrition and energy-use efficiency. Acta Hortic 1227:491-498. https://doi.org/10.17660/ActaHortic.2018.1227.62

10.17660/ActaHortic.2018.1227.62
2

Biswas, T., Parveen, O., Pandey, V. P., Mathur, A., Dwivedi, U. N. (2020) Heavy metal accumulation efficiency, growth and centelloside production in the medicinal herb Centella asiatica (L.) urban under different soil concentrations of cadmium and lead. Ind Crop Prod 157:112948. https://doi.org/10.1016/j.indcrop.2020.112948

10.1016/j.indcrop.2020.112948
3

Bonfill, M., Mangas, S., Moyano, E., Cusido, R. M., Palazón, J. (2011) Production of centellosides and phytosterols in cell suspension cultures of Centella asiatica. Plant Cell Tissue Organ Cult 104:61-67. https://doi.org/10.1007/s11240-010-9804-7

10.1007/s11240-010-9804-7
4

Choi, K. Y., Shawon, M. R. A., Kim, J. K., Yoon, Y. J., Park, S. J., Na, J. K. (2022) Effect of white LED light on the growth of apple seedlings in controlled environment system. Horticulturae 8:692. https://doi.org/10.3390/horticulturae8080692

10.3390/horticulturae8080692
5

Costa, D. F., Yendo, A. C. A., Fleck, J. D., Gosmann, G., Fett-Neto, A. G. (2013) Accumulation of a bioactive triterpene saponin fraction of Quillaja brasiliensis leaves is associated with abiotic and biotic stresses. Plant Physiol Biochem 66:56-62. https://doi.org/10.1016/j.plaphy.2013.02.003

10.1016/j.plaphy.2013.02.00323474431
6

Devkota, A., Jha, P. K. (2010) Effects of different light levels on the growth traits and yield of Centella asiatica. Middle East J Sci Res 5:226-230.

7

James, J. T., Dubery, I. A. (2009) Pentacyclic triterpenoids from the medicinal herb, Centella asiatica (L.) urban. Molecules 14:3922-3941. https://doi.org/10.3390/molecules14103922

10.3390/molecules1410392219924039PMC6255425
8

Li, K., Chen, J., Wei, Q., Li, Q., Lei, N. (2018) Effects of transgenerational plasticity on morphological and physiological properties of stoloniferous herb Centella asiatica subjected to high/low light. Front Plant Sci 9:1640. https://doi.org/10.3389/fpls.2018.01640

10.3389/fpls.2018.0164030487805PMC6246981
9

Müller, V., Lankes, C., Zimmermann, B. F., Noga, G., Hunsche, M. (2013) Centelloside accumulation in leaves of Centella asiatica is determined by resource partitioning between primary and secondary metabolism while influenced by supply levels of either nitrogen, phosphorus or potassium. J Plant Physiol 170:1165-1175. https://doi.org/10.1016/j.jplph.2013.03.010

10.1016/j.jplph.2013.03.01023608744
10

Nawae, W., Yoocha, T., Narong, N., Paemanee, A., Ketngamkum, Y., Romyanon, K., Toojinda, T., Tangphatsornruang, S., Pootakham, W. (2021) Transcriptome sequencing revealed the influence of blue light on the expression levels of light-stress response genes in Centella asiatica. Plos One 16:0260468. https://doi.org/10.1371/journal.pone.0260468

10.1371/journal.pone.026046834843573PMC8629183
11

Prasad, A., Kumari, M., Srivastava, N. K., Mathur, A. K., Mathur, A. (2017) Copper-induced modulation of biomass growth, physiological parameters, bioactive centellosides, and expression of biosynthetic pathway genes in an important medicinal herb, Centella asiatica. J Plant Growth Regul 37:471-480. https://doi.org/10.1007/s00344-017-9745-z

10.1007/s00344-017-9745-z
12

Puig, J., Pauluzzi, G., Guiderdoni, E., Gantet, P. (2012) Regulation of shoot and root development through mutual signaling. Mol Plant 5:974-983. https://doi.org/10.1093/mp/sss047

10.1093/mp/sss04722628542
13

Shawon, M. R. A., Azad, M. O. K., Ryu, B. R., Na, J. K., Choi, K. Y. (2023) The electrical conductivity of nutrient solution influenced the growth, centellosides content and gene expression of Centella asiatica in a hydroponic system. Agriculture 13:2236. https://doi.org/10.3390/agriculture13122236

10.3390/agriculture13122236
14

Shawon, R. A., Ha, S. Y., Lee, T. H., Cao, T. L., Kim, H. C., Bae, J. H., Ku, Y. G. (2021) Influence of shade treatment on plant growth characteristics and spear production in five asparagus (Asparagus officinalis L.) cultivars. Hortic Sci Technol 39:37-48. https://doi.org/10.7235/HORT.20210004

10.7235/HORT.20210004
15

So, E. H., An, J. H., Kim, J. K., Yoon, Y. J., Park, S. J., Na, J. K., Choi, K. Y. (2018) Effect of artificial light sources on the growth of apple rootstock m.9 seedling. J Bio-Env Con 27:341-348. https://doi.org/10.12791/KSBEC.2018.27.4.341

10.12791/KSBEC.2018.27.4.341
16

Song, J. W., Bhandari, S. R., Shin, Y. K., Lee, J. G. (2022) The influence of red and blue light ratios on growth performance, secondary metabolites, and antioxidant activities of Centella asiatica (L.) urban. Horticulturae 8:601. https://doi.org/10.3390/horticulturae8070601

10.3390/horticulturae8070601
17

Thomas, M. T., Kurup, R., Johnson, A. J., Chandrika, S. P., Mathew, P. J., Dan, M., Baby, S. (2010) Elite genotypes/chemotypes, with high contents of madecassoside and asiaticoside, from sixty accessions of Centella asiatica of south India and the andaman islands: for cultivation and utility in cosmetic and herbal drug applications. Ind Crops Prod 32:545-550. https://doi.org/10.1016/j.indcrop.2010.07.003

10.1016/j.indcrop.2010.07.003
18

Zhang, H., Rong, H., Pilbeam, D. (2007) Signalling mechanisms underlying the morphological responses of the root system to nitrogen in Arabidopsis thaliana. J Exp Bot 58:2329-2338. https://doi.org/10.1093/jxb/erm114

10.1093/jxb/erm11417578866
Information
  • Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
  • Publisher(Ko) :강원대학교 농업생명과학대학 농업생명과학연구원
  • Journal Title :Journal of Agricultural, Life and Environmental Sciences
  • Journal Title(Ko) :농업생명환경연구
  • Volume : 37
  • No :1
  • Pages :16-25
  • Received Date : 2024-12-04
  • Revised Date : 2025-02-03
  • Accepted Date : 2025-02-06
  • DOI :https://doi.org/10.22698/jales.20250002
Journal Informaiton Journal of Agricultural, Life and Environmental Sciences Journal of Agricultural, Life and Environmental Sciences
Online Submission & Review System
  • NRF
  • KOFST
  • KISTI Current Status
  • KISTI Cited-by
  • KCI 문헌 유사도 검사
  • orcid
  • open access
  • ccl
Journal Informaiton Journal Informaiton - close