Efficacy of Antagonistic Bacterial Isolates in Suppressing Sclerotinia rot and Promoting Growth in Lettuce

Hyun Seung Kim; Adhikari Mahesh; Hyo Bin Park; Gi Young Kim; In Gyu Lee; Eun Jeong Byeon; Ji Min Woo; Youn Su Lee

doi:10.22698/jales.20220042

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2022 Vol.34, Issue 3 Preview Page Next Page

Research Article

Efficacy of Antagonistic Bacterial Isolates in Suppressing Sclerotinia rot and Promoting Growth in Lettuce 상추 균핵병 억제 및 생장촉진을 위한 길항 세균의 효능

31 December 2022. pp. 433-444

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Abstract

This study was conducted to biochemically characterize and screen the efficacy of antagonistic bacterial isolates in suppressing sclerotinia rot in lettuce crops. A total of 123 bacterial isolates were isolated from soil. In vitro antagonistic screening showed that 13 bacterial isolates were effective against Sclerotinia sclerotiorum and S. minor. In particular, two strains of Bacillus megaterium (HCS-1B and WJR-6B) showed the highest effectiveness against the tested pathogens, with a 75–80% inhibition rate. Additionally, the 13 bacterial isolates were biochemically characterized. Ammonia production, phosphate solubilization, protease production, starch hydrolysis and siderophore production, and HCN production were observed in 13, 3, 11, 9, and 2 bacterial isolates, respectively. Based on the results of the in vitro antagonistic test and biochemical characterization, the two best bacterial isolates (HCS-1B and WJR-6B) were selected for sclerotinia rot suppression and lettuce growth promotion under greenhouse conditions. The lowest incidence rate (0%) of sclerotinia rot was recorded in WJR-6B-treated lettuce pre-inoculated with S. sclerotiorum and in S. sclerotiorum-inoculated lettuce pretreated with HCS-1B. Combination treatment of HCS-1B and WJR-6B increased the leaf area of lettuce by approximately 3-fold (218.4 cm²) compared to the control (75 cm²). In this study, HCS-1B and WJR-6B were identified as potential antagonistic bacterial isolates that inhibit sclerotinia rot. Field testing of these potential bacterial isolates would be a good direction for future studies.

Keywords

Bacillus megaterium

Biochemical characterization

Inhibition

Sclerotinia rot

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Information

Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
Publisher(Ko) :강원대학교 농업생명과학대학 농업생명과학연구원
Journal Title :Journal of Agricultural, Life and Environmental Sciences
Journal Title(Ko) :농업생명환경연구
Volume : 34
No :3
Pages :433-444
Received Date : 2022-11-29
Revised Date : 2022-12-14
Accepted Date : 2022-12-15
DOI :https://doi.org/10.22698/jales.20220042

Journal of Agricultural, Life and Environmental SciencesISSN:2233-8322(Print) 2508-870X(Online)강원대학교 농업생명과학대학 농업생명과학연구원

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