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2019 Vol.31, Issue 3

Research Article

Development of Species-specific Primer Sets for Detecting Cirsium setidens and Silybum marianum using Real-time PCR Assay and Their Application in Commercial Food Products
J. An1
,
G. S. Kim1
,
C. S. Jang2*
1Master Students, Plant Genomics Laboratory, Department of Applied Plant Sciences, Kangwon National University
2Professor, Plant Genomics Laboratory, Department of Applied Plant Sciences, Kangwon National University
* Corresponding Author
31 December 2019. pp. 81-93
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Abstract

Cirsium setidens and Silybum marianum are medicinal plants that have been recently recognized as highly valuable sources of herbal drugs in Korea as well as worldwide. However, molecular markers for detecting these species in commercial food products have not yet been reported. In this study, we developed six species-specific primer sets for C. setidens and S. marianum using quantitative real-time PCR (qPCR) analysis, based on the single nucleotide polymorphisms (SNPs) of four chloroplast genes (ndhA, ndhF, rbcL and ycf1). The developed primer pairs showed correlation coefficients for their target species only (R2 > 0.99), and their efficiencies and slopes were in the range of 93－103% and -3.23 to -3.49, respectively. A total of 12 commercial food products including seven for C. setidens and 5 for S. marianum were tested to evaluate the feasibility of the developed primer sets. We found that C. setidens was present in all the seven C. setidens commercial products, while presence of S. marianum was confirmed in four of five S. marianum products. Therefore, the developed species-specific primer sets could prove a useful tool for detecting the presence of C. setidens and S. marianum in commercial food products.

Keywords
Cirsium setidens
Real-time PCR
Silybum marianum
SNP
Species-specific DNA markers
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Information
  • Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
  • Publisher(Ko) :None
  • Journal Title :Journal of Agricultural, Life and Environmental Sciences
  • Journal Title(Ko) :농업생명환경연구
  • Volume : 31
  • No :3
  • Pages :81-93
  • Received Date : 2019-11-15
  • Revised Date : 2019-11-30
  • Accepted Date : 2019-11-30
  • DOI :https://doi.org/10.22698/jales.20190010
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