Characterization of Bacteria Isolated from the Neungam Carbonate Hot Springs

Woo-Yang Jeong; Sang-Jae Lee; Yong-Jik Lee

doi:10.22698/jales.20230047

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2023 Vol.35, Issue 4 Preview Page Next Page

Research Article

Characterization of Bacteria Isolated from the Neungam Carbonate Hot Springs 능암 탄산 온천에서 분리한 세균의 특성 분석

31 December 2023. pp. 585-592

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Abstract

In this study, the hot spring water of the Neungam Carbonate Hot Springs in Korea was used to isolate Bacillus strains using marine agar as the medium, and three strains were isolated through single colony isolation. The growth pH and temperature of the isolated strains and their growth potential in complex media were analyzed. Based on strain identification, three strains were confirmed to be members of the Bacillaceae family, but from different genera, and included Bacillus tequilensis, Cytobacillus oceanisediminis, and Caldibacillus hisashii. In addition, cultured media were tested for the ability of the strains to produce extracellular hydrolysis enzymes to determine whether the strains could be used in the cosmetics or food industry, and two strains were confirmed to exhibit extracellular protease activity. Finally, to optimize the medium composition for mass cultivation of the M510 strain, the ability to use various carbon sources was analyzed and 20 types of carbon sources that could be used for carbohydrate metabolism were confirmed. The results of this study can be used as basic research data to secure domestic genetic resources and optimize media for isolated strains.

Keywords

Biological process

Carbohydrate

Enzyme

Hot spring

Identification

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Information

Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
Publisher(Ko) :강원대학교 농업생명과학대학 농업생명과학연구원
Journal Title :Journal of Agricultural, Life and Environmental Sciences
Journal Title(Ko) :농업생명환경연구
Volume : 35
No :4
Pages :585-592
Received Date : 2023-11-30
Revised Date : 2023-12-06
Accepted Date : 2023-12-07
DOI :https://doi.org/10.22698/jales.20230047

Journal of Agricultural, Life and Environmental Sciences ISSN:2233-8322(Print) 2508-870X(Online) 농업생명환경연구

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