All Issue

2024 Vol.36, Issue 3 Preview Page

Research Article

Effects of Antagonistic on Acidovorax citrulli in Field Experiments

길항미생물을 활용한 Acidovorax citrulli의 생물적 방제 포장 실증시험

Won Jun Chang1
,
Hyun Seung Kim2
,
Yun Seok Kim3
,
Sang Woo Kim4
,
Eun Jeong Byeon5
,
Ji Min Woo5
,
In Kyu Lee6
,
Youn Su Lee7*
장 원준1
,
김 현승2
,
김 윤석3
,
김 상우4
,
변 은정5
,
우 지민5
,
이 인규6
,
이 윤수7*
1MS student, Interdisciplinary Program in Smart Agriculture, Kangwon National University, Chuncheon 24341, Korea
2Ph. D student, Interdisciplinary Program in Smart Agriculture, Kangwon National University, Chuncheon 24341, Korea
3Ph. D student, Department of Applied Plant Sciences, Kangwon National University, Chuncheon 24341, Korea
4Ph. D, Department of Applied Plant Sciences, Kangwon National University, Chuncheon 24341, Korea
5MS, Department of Applied Plant Sciences, Kangwon National University, Chuncheon 24341, Korea
6MS, Department of Life Sciences, Pohang University of Science and Technology, Pohang 37673, Korea
7Professor, Department of Applied Plant Sciences, Kangwon National University, Chuncheon 24341, Korea
1강원대학교 스마트농업융합학과 석사과정
2강원대학교 스마트농업융합학과 박사과정
3강원대학교 식물자원응용과학과 박사과정
4강원대학교 식물자원응용과학과 박사
5강원대학교 식물자원응용과학과 석사
6포항공과대학교 생명과학과 석사
7강원대학교 식물자원응용과학과 교수
*Corresponding Author
30 September 2024. pp. 243-253
PDF XML Citation
Abstract

Acidovorax citrulli is a plant pathogenic bacterium that causes bacterial fruit blotch (BFB) disease in cucurbit plants worldwide. This pathogen can reside not only in the fruit but also in seeds, leading to infections in subsequent generations. Because research on A. citrulli has primarily been conducted through in vitro experiments, few studies have replicated real agricultural environments or investigated the effects on fruits and seeds. In this study, antagonistic bacteria (TIPL-6-1B, TIPL-8-1B) with high inhibitory ability against A. citrulli (140) were selected from in vitro experiments and used in field validation tests. Plants in open fields and greenhouses were inoculated with the pathogen (140) and treated with antagonistic bacteria (TIPL-6-1B, TIPL-8-1B). The incidence of BFB in fruits and the rate of infected seeds were significantly reduced in plants treated with the antagonistic bacteria, compared to those in the negative control group. These results suggest that an infection of A. citrulli strain (140) can be effectively suppressed by Bacillus velezensis strains (TIPL-6-1B, TIPL-8-1B) in actual agricultural settings, thereby benefiting watermelon cultivation.

Keywords
Antagonistic bacteria
Bacterial fruit blotch
Field experiment
Seed-detection
References
1

Adhikari, M., Kim, S. W., Um, Y. H., Kim, H. S., Lee, S. C., Song, J. Y., Kim, H. G., Lee, Y. S. (2017) Biological control of bacterial fruit blotch of watermelon pathogen (Acidovorax citrulli) with rhizosphere associated bacteria. The Plant Pathology Journal 33:170.

10.5423/PPJ.OA.09.2016.0187
2

Burdman, S., Walcott, R. (2012) Acidovorax citrulli: generating basic and applied knowledge to tackle a global threat to the cucurbit industry. Mol Plant Pathol 13:805-815.

10.1111/j.1364-3703.2012.00810.x
3

Cai, X., Yan, F., Lin, Y., Hu, F. (2009) Isolation and screening of the antagonistic and endophytic bacteria against Acidovorax avenae subsp. citrulli. Journal of Fujian Agriculture and Forestry University (Natural Science Edition) 38:465-470.

4

Carvalho, F. C., Santos, L. A., Dias, R. C., Mariano, R. L., Souza, E. B. (2013) Selection of watermelon genotypes for resistance to bacterial fruit blotch. Euphytica 190:169-180.

10.1007/s10681-012-0766-1
5

Cho, M. S., Park, D. H., Ahn, T.-Y., Park, D. S. (2015) Rapid and specific detection of Acidovorax avenae subsp. citrulli using SYBR green-based real-time PCR amplification of the YD-repeat protein gene. Microbiol Biotechnol 25:1401-1409.

10.4014/jmb.1502.02029
6

Dutta, B., Ha, Y., Lessl, J., Avci, U., Sparks, A., Johnson, K., Walcott, R. (2015) Pathways of bacterial invasion and watermelon seed infection by Acidovorax citrulli. Mol Plant Pathol 64:537-544.

10.1111/ppa.12307
7

Fan, H., Zhang, Z., Li, Y., Zhang, X., Duan, Y., Wang, Q. (2017) Biocontrol of bacterial fruit blotch by Bacillus subtilis 9407 via surfactin-mediated antibacterial activity and colonization. Front Microbiol 8:1973.

10.3389/fmicb.2017.01973
8

Gao, T., Hao, F., Yang, D., Bie, Z., Li, G. (2017) Oxalic acid produced by Aspergillus niger Y-1 is effective for suppression of bacterial fruit blotch of watermelon seedlings. Biol Control 112:28-33.

10.1016/j.biocontrol.2017.06.001
9

Hopkins, D. L., Thompson, C. (2002) Evaluation of Citrullus sp. germ plasm for resistance to Acidovorax avenae subsp. citrulli. Plant Di 86:61-64

10.1094/PDIS.2002.86.1.61
10

Isakeit, T., Black, M., Barnes, L., Jones, J. (1997) First report of infection of honeydew with Acidovorax avenae subsp. citrulli. Plant Di 81:694-694.

10.1094/PDIS.1997.81.6.694C
11

Jiang, C.-H., Wu, F., Yu, Z.-Y., Xie, P., Ke, H.-J., Li, H.-W., Yu, Y.-Y., Guo, J.-H. (2015) Study on screening and antagonistic mechanisms of Bacillus amyloliquefaciens 54 against bacterial fruit blotch (BFB) caused by Acidovorax avenae subsp. citrulli. Microbial Res 170:95-104.

10.1016/j.micres.2014.08.009
12

Jin, M., Zhao, Z., Gao, Y., Yang, N. (2014) Occurrence and control measures of watermelon fruit blotch in Litong district in Wuzhong municipality. Ningxia J Agri Fores (China) 55:33-34.

13

Kim, K. Y., Park, H. B., Adhikari, M., Kim, H. S., Byeon, E. J., Lee, I. K., Lee, Y. S. (2022) Selection and characterization of antagonistic microorganisms for biological control of Acidovorax citrulli causing fruit rot in watermelon. J Plant Prot Res 28:69-81.

10.5423/RPD.2022.28.2.69
14

Langston Jr, D., Walcott, R., Gitaitis, R., Sanders Jr, F. (1999) First report of a fruit rot of pumpkin caused by Acidivorax avenae subsp. citrulli in Georgia. Plant Dis 83:199-199.

10.1094/PDIS.1999.83.2.199B
15

Lessl, J., Fessehaie, A., Walcott, R. (2007) Colonization of female watermelon blossoms by Acidovorax avenae ssp. citrulli and the relationship between blossom inoculum dosage and seed infestation. J Phytopathol 155:114-121.

10.1111/j.1439-0434.2007.01204.x
16

Martin, H., O'Brien, R., Abbott, D. (1999) First report of Acidovorax avenae subsp. citrulli as a pathogen of cucumber. Plant Dis 83:965-965.

10.1094/PDIS.1999.83.10.965D
17

Rahimi-Midani, A., Kim, J.-O., Kim, J. H., Lim, J., Ryu, J.-G., Kim, M.-K., Choi, T.-J., (2020) Potential use of newly isolated bacteriophage as a biocontrol against Acidovorax citrulli. Arch Microbiol 202: 377-389.

10.1007/s00203-019-01754-5
18

Rane, K. K., Latin, R. X. (1992) Bacterial fruit blotch of watermelon: Association of the pathogen with seed. Plant Dis 76:509-512.

10.1094/PD-76-0509
19

Reva, O. N., Swanevelder, D. Z., Mwita, L. A., Mwakilili, A. D., Muzondiwa, D., Joubert, M., Chan, W. Y., Lutz, S., Ahrens, C. H., Avdeeva, L. V. (2019) Genetic, epigenetic and phenotypic diversity of four Bacillus velezensis strains used for plant protection or as probiotics. Front Microbiol 10:2610.

10.3389/fmicb.2019.02610
20

Schaad, N. W., Sowell Jr, G., Goth, R., Colwell, R., Webb, R. (1978) Pseudomonas pseudoalcaligenes subsp. citrulli subsp. nov. Int J Syst Evol Microbiol 28:117-125.

10.1099/00207713-28-1-117
21

Somodi, G. C., Jones, J., Hopkins, D., Stall, R., Kucharek, T., Hodge, N., Watterson, J. (1991) Occurrence of a bacterial watermelon fruit blotch in Florida. Plant Dis 75:53-51.

10.1094/PD-75-1053
22

Walcott, R., Fessehaie, A., Castro, A. (2004) Differences in pathogenicity between two genetically distinct groups of Acidovorax avenae subsp. citrulli on cucurbit hosts. J Phytopathol 152:277-285.

10.1111/j.1439-0434.2004.00841.x
Information
  • Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
  • Publisher(Ko) :강원대학교 농업생명과학대학 농업생명과학연구원
  • Journal Title :Journal of Agricultural, Life and Environmental Sciences
  • Journal Title(Ko) :농업생명환경연구
  • Volume : 36
  • No :3
  • Pages :243-253
  • Received Date : 2024-06-05
  • Revised Date : 2024-07-19
  • Accepted Date : 2024-08-26
  • DOI :https://doi.org/10.22698/jales.20240020
Journal Informaiton Journal of Agricultural, Life and Environmental Sciences Journal of Agricultural, Life and Environmental Sciences
Online Submission & Review System
  • NRF
  • KOFST
  • KISTI Current Status
  • KISTI Cited-by
  • KCI 문헌 유사도 검사
  • orcid
  • open access
  • ccl
Journal Informaiton Journal Informaiton - close