Characteristics of the Yeast Starmerella vaccinii Isolated from Dianthus chinensis

Jeong-Ah Yoon; Jung-Un Kim; So-Hee Shin; Myoung-Dong Kim

doi:10.22698/jales.20210002

All Issue

2021 Vol.33, Issue 1 Preview Page Next Page

Research Article

Characteristics of the Yeast Starmerella vaccinii Isolated from Dianthus chinensis 패랭이꽃으로부터 분리한 효모 Starmerella vaccinii 균주 특성

31 March 2021. pp. 16-24

PDF XML

Abstract

Two hundred and fifty-eight strains of yeast were isolated from wild flowers collected in Chuncheon, Gangwon Province, Korea. The MBY/L7303 strain from Dianthus chinensis showed remarkable growth in medium supplemented with glucose, xylose, cellobiose, and galactose as the carbon source. This strain was identified as Stamerellera vaccinii, and its 26S rRNA gene sequence was registered in GenBank (MW465704). The S. vaccinii MBY/L7303 utilized glucose, cellobiose, sucrose, galactose, xylose, xylan, and starch as carbon sources and did not produce ethanol. The strain grew to a maximum cell density (OD₆₀₀) of 21.93 ± 0.09 in the cellobiose-supplemented medium. The highest specific growth rate was 0.41 ± 0.00 1/h at pH 5.0 and 30°C. These results serve as valuable information for the potential industrial application of S. vaccinii.

Keywords

Dianthus chinensis

Starmerella vaccinii

Wild flowers

Yeast

References

Choi, D. H., Choi, Y. H., Yeo, S. H., Kim, M. D. (2016) Isolation and characterization of Saccharomyces cerevisiae from nuruk for production of ethanol from maltose. Microbiol Biotechnol Lett 44:34-39. 10.4014/mbl.1510.10008

Choi, D. H., Park, E. H., Kim, M. D. (2017) Isolation of thermotolerant yeast Pichia kudriavzevii from nuruk. Food Sci Biotechnol 26:1357-1362. 10.1007/s10068-017-0155-630263670PMC6049779

Davey, K. G., Chant, P. M., Downer, C. S., Campbell, C. K., Warnock, D. W. (1995) Evaluation of the AUXACOLOR system, a new method of clinical yeast identification. J Clin Pathol 48:807-809. 10.1136/jcp.48.9.8077490311PMC502866

Duncan, D. B. (1955) Multiple range and multiple F tests. Biometrics 11:1-42. 10.2307/3001478

Ha, S. J., Wei, Q., Kim, S. R., Galazka, J. M., Cate, J., Jin, Y. S. (2011) Cofermentation of cellobiose and galactose by an engineered Saccharomyces cerevisiae strain. Appl Environ Microbiol 77:5822-5825. 10.1128/AEM.05228-1121705527PMC3165266

Han, S. M., Lee, S. Y., Kim, H. K., Lee, J. S. (2017) Characterization of wild yeasts isolated from leaves obtained from Mt. Daedun and Mt. Chilgap, Korea. Kor J Mycol 45:31-42.

Hansen, E. C. (1883) Undersøgelser over alkoholgjaersvampenes fysiologi og morfologi II Om asko sporedannelse hos slaegten Saccharomyces. Medd Carlsberg Lab 2:29-86

Hyun, S. H., Han, S. M., Lee, J. S. (2014) Characteristics and physiological functionalities of unrecorded yeasts from wild flowers of Seonyudo in Jeollabuk-do, Korea. Microbiol Biotechnol Lett 42:402-406. 10.4014/kjmb.1409.09001

Hyun, S. H., Mun, H. Y., Lee, H. B., Kim, H. K., Lee, J. S. (2013) Isolation of yeasts from wild flowers in Gyonggi-do province and Jeju island in Korea and the production of anti-gout xanthine oxidase inhibitor. Microbiol Biotechnol Lett 41:383-390. 10.4014/kjmb.1309.09002

Jung, E. H., Bae, Y. W., Kwun, S. Y., Park, E. H., Kim, M. D. (2019) Isolation of xylitol-producing thermotolerant yeast Millerozyma farinosa from nuruk. Microbiol Biotechnol Lett 47:530-535. 10.4014/mbl.1902.02006

Kumar, S., Stecher, G., Li, M., Knyaz, C., Tamura, K. (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35:1547-1549. 10.1093/molbev/msy09629722887PMC5967553

Lee, J. S., Yi, S. H., Kwon, S. J., Ahn, C., Yoo, J. Y. (1997) Enzyme activities and physiological functionality of yeasts from traditional Meju. Kor J Appl Microbiol Biotechnol 25:448-453.

Lee, S. H. (2005) Development of bactericidal yeast strain by expressing the leucocin a gene on the cell surface of Saccharomyces cerevisiae. Kor J Life Sci 15:923-927. 10.5352/JLS.2005.15.6.923

Lee, S. K., Cho, N. J., Park, W. J. (2003) Development of the formula for natural bread-making starter. J Korean Soc Food Sci Nutr 32:1245-1252. 10.3746/jkfn.2003.32.8.1245

Lee, T. S. (1972) The role of useful yeasts in the soy sauce mash. Korean J Microbiol 10:87-92.

Lopes, M. R., Lara, C. A., Moura, M. E., Uetanabaro, A. P. T., Morais, P. B., Vital, M. J., Rosa, C. A. (2018) Characterisation of the diversity and physiology of cellobiose-fermenting yeasts isolated from rotting wood in Brazilian ecosystems. Fungal Biol 122:668-676. 10.1016/j.funbio.2018.03.00829880202

Oslan, S. N., Salleh, A. B., Abd Rahman, R. N. Z. R., Basri, M., Chor, A. L. T. (2012) Locally isolated yeasts from Malaysia: identification, phylogenetic study and characterization. Acta Biochim Pol 59:225-229. 10.18388/abp.2012_214222577620

Park, I. C., Kim, J. S., Jung, J., Yoo, J. H. (2013) Characterization of phosphate solubilizing yeasts from Korean traditional fermented foods. Kor J Mycol 41:218-224. 10.4489/KJM.2013.41.4.218

Rajkowska, K., Kunicka-Styczyńska, A., Rygala, A. (2012) Probiotic activity of Saccharomyces cerevisiae var. boulardii against human pathogens. Food Technol Biotechnol 50:230-36.

Reynolds, D. R., Taylor, J. W. (1993) The fungal holomorph: mitotic, meiotic and pleomorphic speciation in fungal systematics. CAB International 225-233.

Rollero, S., Bloem, A., Ortiz-Julien, A., Camarasa, C., Divol, B. (2018) Fermentation performances and aroma production of non-conventional wine yeasts are influenced by nitrogen preferences. FEMS Yeast Res 18:1-11. 10.1093/femsyr/foy05529741618

Saitou, N., Nei, M. (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406-425.

Santos, A. R. O., Leon, M. P., Barros, K. O., Freitas, L. F., Hughes, A. F., Morais, P. B., Rosa, C. A. (2018) Starmerella camargoi fa, sp. nov., Starmerella ilheusensis fa, sp. nov., Starmerella litoralis fa, sp. nov., Starmerella opuntiae fa, sp. nov., Starmerella roubikii fa, sp. nov. and Starmerella vitae fa, sp. nov., isolated from flowers and bees, and transfer of related Candida species to the genus Starmerella as new combinations. Int J Syst Evol Microbiol 68:1333-1343. 10.1099/ijsem.0.00267529498615

Sipiczki, M. (2010) Candida stigmatis sp. nov., a new anamorphic yeast species isolated from flowers. FEMS Yeast Res 10:362-365. 10.1111/j.1567-1364.2009.00574.x19788562

Sipiczki, M. (2015) Starmerella syriaca fa, sp. nov., an osmotolerant yeast species isolated from flowers in Syria. Antonie van Leeuwenhoek 107:847-856. 10.1007/s10482-015-0377-725583140

Soccol, C. R., Vandenberghe, L. P. D. S., Spier, M. R., Medeiros, A. B. P., Yamaguishi, C. T., Lindner, J. D. D., Thomaz-Soccol, V. (2010) The potential of probiotics: a review. Food Technol Biotechnol 48:413-434.

Toma, M. M., Raipulis, J., Kalnina, I., Rutkis, R. (2005) Effect of probiotic yeast on genotoxicity. Food Technol Biotechnol 43:301-305.

Yi, J. H., Heo, N. K., Choi, B. G., Park, E. H., Kwun, S. Y., Kim, M. D., Baek, S. Y. (2014) Isolation of fibrinolytic yeasts from Korean traditional fermented soybean. Microbiol Biotechnol Lett 42:184-189. 10.4014/kjmb.1403.03006

Yoo, B. H., Park, E. H., Kim, M. D. (2016) Enhanced resistance to lactic acid by laboratory adaptive evolution of Saccharomycopsis fibuligera. Microbiol Biotechnol Lett 44:488-492. 10.4014/mbl.1608.08001

Yoon, J. A., Do, Y. E., Park, E. H., Bae, Y. W., Kim, M. D. (2020) Isolation of ethanol-producing thermotolerant yeast Hanseniaspora opuntiae from Senecio cruentus. Microbiol Biotechnol Lett 48:179-184. 10.4014/mbl.2003.03003

Information

Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
Publisher(Ko) :강원대학교 농업생명과학대학 농업생명과학연구원
Journal Title :Journal of Agricultural, Life and Environmental Sciences
Journal Title(Ko) :농업생명환경연구
Volume : 33
No :1
Pages :16-24
Received Date : 2021-02-02
Revised Date : 2021-03-21
Accepted Date : 2021-03-24
DOI :https://doi.org/10.22698/jales.20210002

Journal of Agricultural, Life and Environmental Sciences ISSN:2233-8322(Print) 2508-870X(Online) 농업생명환경연구

All Issue