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2024 Vol.36, Issue 4 Preview Page

Research Article

Antioxidant Activity of Aceriphyllum rossi Leaf Extracts using Different Solvents
Jaehee Lee1*
,
Jaehyung Yi1
,
Ki-yeon Lee1
,
Eunha Jang2
,
Youngho Seo3
,
Ki-seon Kim3
1Agricultural researcher, Gangwon State Agricultural Research & Extension Services Agricultural Environmental Research Section
2Agricultural senior researcher, Gangwon State Agricultural Research & Extension Services Horticultural Research Section
3Agricultural senior researcher, Gangwon State Agricultural Research & Extension Services Agricultural Environmental Research Section
*Corresponding Author
31 December 2024. pp. 375-386
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Abstract

The aim of this study was to evaluate the antioxidant potential of Aceriphyllum rossii leaf (ARL) extracts obtained using water and 10%, 30%, 50%, and 70% ethanol solutions as extraction solvents. These different extracts obtained from ARL, traditionally recognized for its health benefits, were analyzed to determine the effect of different solvent concentrations on the antioxidant extraction efficiency and antioxidant activities of these extracts. Antioxidant assays, including the DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2’-azinobis-[3-ethylbenzothiazoline-6-sulfonic acid]) radical scavenging assays and ORAC (oxygen radical absorbance capacity) and FRAP (ferric-reducing antioxidant power) assays, were performed to quantify the free radical scavenging capacity of each extract, alongside the measurements of total polyphenol and flavonoid contents. The results revealed that ethanol extracts consistently exhibited higher antioxidant activities and polyphenol contents than those of water extracts, with the 50% ethanol extract achieving the highest total polyphenol (381.27 mg/g) and flavonoid contents (176.53 mg/g). Both 30% and 50% ethanol extracts demonstrated superior free radical scavenging activities, suggesting mid-range ethanol concentrations as optimal for efficiently extracting antioxidant compounds from ARL. Notably, the 30% ethanol extract maintained high antioxidant efficacy while using a lower ethanol concentration, which provides a cost-effective solution for industrial applications by reducing ethanol recovery and solvent costs. Therefore, the 30% ethanol extract presents a practical choice for large-scale production, with potential applications as a natural antioxidant in functional foods and cosmetics. These findings highlight the importance of ARL as a promising source of antioxidants, emphasizing that optimal solvent selection can enhance both economic feasibility and functional benefits. Future studies are recommended to explore the in vivo efficacy and safety of ethanol-based ARL extracts, aiming to expand their potential as a versatile ingredient in health-promoting products.

Keywords
ABTS
Aceriphyllum rossii
Antioxidant activity
DPPH
Extraction solvent
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Information
  • Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
  • Publisher(Ko) :None
  • Journal Title :Journal of Agricultural, Life and Environmental Sciences
  • Journal Title(Ko) :농업생명환경연구
  • Volume : 36
  • No :4
  • Pages :375-386
  • Received Date : 2024-10-25
  • Revised Date : 2024-10-30
  • Accepted Date : 2024-11-04
  • DOI :https://doi.org/10.22698/jales.20240028
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