Anti-obesity Effect of Protein Hydrolysate Derived from Japanese Rhinoceros Beetle (Allomyrina dichotoma) Larvae

Gang Hoon Lee; Hye young Jin; Tai Hyeon Ahn; Jiyeon Sim; Jun Ho Lee; Tae Yun Choi; Jang Woo Park; Mi Jeong Kim; Mi Hye Kim; Sang Woo Kim; Saeyoull Cho

doi:10.22698/jales.20240029

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2024 Vol.36, Issue 4 Preview Page Next Page

Research Article

Anti-obesity Effect of Protein Hydrolysate Derived from Japanese Rhinoceros Beetle (Allomyrina dichotoma) Larvae

31 December 2024. pp. 387-395

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Abstract

In this study, the anti-obesity effects of protein hydrolysates derived from Japanese rhinoceros beetle (Allomyrina dichotoma) larvae were investigated. Four different hydrolysate fractions were assessed: Flavourzyme 10 kDa, Flavourzyme 30 kDa, Protana 10 kDa, and Protana 30 kDa. Cytotoxicity assays revealed that over 80% of cells remained viable in the presence of up to 250 µg/mL hydrolysate. Among the tested samples, Flavourzyme 10 kDa (200 µg/mL) was the most effective in suppressing the expression of sterol regulatory element-binding transcription factor-1 (SREBP-1) and fatty acid synthase (FAS), key proteins involved in lipid synthesis in 3T3-L1 adipocytes. Oil Red O staining further confirmed that Flavourzyme 10 kDa (200 µg/mL) significantly inhibited lipid accumulation during adipocyte differentiation. These results suggest that the Flavourzyme 10 kDa hydrolysate at 200 µg/mL holds promise as a functional anti-obesity ingredient in pet food products.

Keywords

Adipocytes

Anti-Obesity

Hydrolysate

Japanese rhinoceros beetle

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Information

Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
Publisher(Ko) :None
Journal Title :Journal of Agricultural, Life and Environmental Sciences
Journal Title(Ko) :농업생명환경연구
Volume : 36
No :4
Pages :387-395
Received Date : 2024-10-24
Revised Date : 2024-11-04
Accepted Date : 2024-11-09
DOI :https://doi.org/10.22698/jales.20240029

Journal of Agricultural, Life and Environmental Sciences ISSN:2233-8322(Print) 2508-870X(Online)

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