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2024 Vol.36, Issue 3 Preview Page

Research Article

Protective Effects of Kaempferol, Quercetin, and Its Glycosides against Hydrogen Peroxide-Induced Oxidative Stress in SH-SY5Y Neuronal Cells

Kaempferol, Quercetin 및 그 배당체의 Hydrogen Peroxide에 의한 신경세포의 산화적 스트레스 보호 효과

Ji Hyun Kim1
,
Jine Shang Choi2*
,
Eun Ju Cho3*
김 지현1
,
최 진상2*
,
조 은주3*
1Associate professor, Department of Food Science and Nutrition, Gyeongsang National University, Jinju 52828, Korea
2Professor, Department of Food Science and Technology, Gyeongsang National University, Jinju 52828, Korea
3Professor, Department of Food Science and Nutrition, Pusan National University, Busan 46241, Korea
1경상국립대학교 식품영양학과 부교수
2경상국립대학교 식품공학과 교수
3부산대학교 식품영양학과 교수
*Corresponding Author
30 September 2024. pp. 278-288
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Abstract

Oxidative stress in the brain is a well-known cause of neurodegenerative diseases including Alzheimer’s disease. In the present study, we investigated the protective effects of flavonoids such as kaempferol (K), quercetin (Q), kaempferol-3-O-glucoside (KG), and quercetin-3-β-D-glucoside (QG) from oxidative stress induced by H2O2 in SH-SY5Y neuronal cells. Cell viability was confirmed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. To examine the neuroprotective mechanism, we measured the expression of apoptosis-related proteins such as caspase-3, B-cell lymphoma 2 (Bcl-2), and Bcl-2-associated X protein (Bax) using western blotting. Compared with normal cells, treatment with H2O2 significantly decreased cell viability, whereas treatment with the four flavonoids increased cell viability compared with that of H2O2-treated control cells. In particular, treatment with Q led to an increase in cell viability from 65.32% to 94.37%, which was the strongest protective effect observed among the flavonoids. In addition, H2O2-treated cells induced apoptosis by upregulating cleaved caspase-3 and Bax and downregulating Bcl-2. However, flavonoid treatment attenuated neuronal apoptosis by regulating apoptosis-related factors. Kaempferol-3-O-glucoside attenuated apoptosis by inhibiting Bax expression. Compared with the H2O2-treated control group, treatment with Q and QG downregulated the expression of pro-apoptotic proteins, such as cleaved caspase-3 and Bax. The results of this study indicate that the four flavonoids, K, KG, Q, and QG, attenuate H2O2-induced oxidative stress by regulating apoptosis-related factors in SH-SY5Y neuronal cells, suggesting that they are promising agents for oxidative stress-related neurodegenerative diseases.

Keywords
Kaempferol
Kaempferol-3-O-glucoside
Oxidative stress
Quercetin
Quercetin-3-β-D-glucoside
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Information
  • Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
  • Publisher(Ko) :강원대학교 농업생명과학대학 농업생명과학연구원
  • Journal Title :Journal of Agricultural, Life and Environmental Sciences
  • Journal Title(Ko) :농업생명환경연구
  • Volume : 36
  • No :3
  • Pages :278-288
  • Received Date : 2024-07-29
  • Revised Date : 2024-09-09
  • Accepted Date : 2024-09-10
  • DOI :https://doi.org/10.22698/jales.20240022
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