Ameliorative Effects of Pyrus pyrifolia (Burm.f) Nakai Fruit Extract on DSS-Induced Colitis in C57BL/6 Mice

Mi-Ra Lee; Bohye Kim; Nam-Gook Kim; Hee-Joung Kim; Moonjin Ra; Bong-Hwan Chung; Yongjun Lee

doi:10.22698/jales.20210017

All Issue

2021 Vol.33, Issue 3S Preview Page Next Page

Research Article

Ameliorative Effects of Pyrus pyrifolia (Burm.f) Nakai Fruit Extract on DSS-Induced Colitis in C57BL/6 Mice DSS로 유도한 마우스의 대장염에 대한 돌배 추출물의 개선효과

31 October 2021. pp. 166-175

PDF XML

Abstract

Pyrus pyrifolia (Burm.f) Nakai fruit has been used as a traditional medicine for fever, cough, asthma, and rheumatic arthritis. This study aimed to investigate the anti-inflammatory effects of P. pyrifolia Nakai fruit extract (PPE) against DSS-induced colitis in mice. PPE—water extract (PWE), ethanol extract (PEE), or fermented extract (PFE)—was administered for 30 days. After 21 days of PPE treatment, ulcerative colitis was induced by oral administration of 2.5% DSS for 5 days. The mice were divided into seven groups: one normal control (NC) group and six groups with different treatments—DSS, DSS + 100 mg/kg PWE (PWL), DSS + 400 mg/kg PWE (PWH), DSS + 100 mg/kg PEE (PEL), DSS + 400 mg/kg PEE (PEH), DSS+ PFE (1:4 dilution, PFE), respectively. Mice treated with DSS showed severe bloody stools, diarrhea, and weight loss. Colon tissue in DSS group exhibited a marked decrease in its length. However, PPE treatment improved the disease activity index score and colon length. PPE alleviated DSS-induced inflammatory colon lesions. The level of IL-1β, a proinflammatory cytokine, was significantly decreased in PFE group. Moreover, colonic myeloperoxidase activity was significantly reduced in all PPE-treated groups, except in PWL group. These results show that PPE can be used for developing effective and safe functional health products for inflammatory bowel disease.

Keywords

Colitis

Dextran sulfate sodium

Inflammatory bowel disease

Pyrus pyrifolia Nakai

References

Berberat, P. O., A-Rahim, Y. I., Yamashita, K., Warny, M. M., Csizmadia, E., Robson, S. C., Bach, F. H. (2005) Heme oxygenase-1-generaed biliverdin ameliorates experimental murine colitis. Inflamm Bowel Dis 11:350-359. 10.1097/01.MIB.0000164017.06538.8a15803024

Choe, J. Y., Lee, S. P. (2019) High production of GABA in Pyrus ussuriensis Maximowicz fruit extract by mixed fermentation of lactic acid bacteria. Korea J Food Preserv 26:642-649. 10.11002/kjfp.2019.26.6.642

Currò, D., Ianiro, G., Pecere, S., Bibbò, S., Cammarota, G. (2017) Probiotics, fibre and herbal medicinal products for functional and inflammatory bowel disorders. Br J Pharmacol 174:1426-1449. 10.1111/bph.1363227696378PMC5429330

Ferenczi, S., Szegi, K., Winkler, Z., Barna, T., Kovacs, K. J. (2016) Oligomannan prebiotic attenuates immunological, clinical and behavioral symptoms in mouse model of inflammatory bowel disease. Sci Rep 6:34132. 10.1038/srep3413227658624PMC5034233

Goyal, N., Rana, A., Ahlawat, A., Bijjem, K. R. V., Kumar, P. (2014) Animal models of inflammatory bowel disease: A review. Inflammopharmacology 22:219-233. 10.1007/s10787-014-0207-y24906689

Jeon, J. H., Yang, J. Y., Lee, H. S. (2012) Acaricidal activities of materials derived from pyrus ussuriensis fruits against stored food mites. J Food Prot 75:1258-1262. 10.4315/0362-028X.JFP-12-04022980009

Jin, B. R., Chung, K. S., Cheon, S. Y., Lee, M., Hwang, S., Hwang, S. N., Rhee, K. J., An, H. J. (2017) Rosmarinic acid suppresses colonic inflammation in dextran sulphate sodium (DSS)-induced mice via dual inhibition of NF-κB and STAT3 activation. Sci Rep 7:46252. 10.1038/srep4625228383063PMC5382778

Jin, S., Sato, N. (2003) Benzoquinone, the substance essential for antibacterial activity in aqueous extracts from succulent young shoots of the pear Pyrus spp. Phytochemistry 62:101-107. 10.1016/S0031-9422(02)00444-2

Kim, B. H., Lee, W. J., Sanjel, B., Cho, K. H., Son, Y. K., Park, H. Y., Kim, S. Y., Shim, W. S. (2019) Extracts of the leaves of Pyrus ussuriensis Maxim. Alleviate itch sensation via TSLP-dependent manner in mouse models of atopic dermatitis. Physiol Behav 210:112624. 10.1016/j.physbeh.2019.11262431325512

Klebanoff, S. J. (2005) Myeloperoxidase: Frend and foe. J Leukoc Biol 77:598-625. 10.1189/jlb.120469715689384

Korea Association for the Study of Intestinal Disease (KASID) (2020) Inflammatory bowel disease fact sheet in Korea.

Lee, Y. G., Cho, J. Y., Park, J., Lee, S. H., Kim, W. S., Park, K. H., Moon, J. H. (2013) Large-scale isolation of highly pure malaxinic acid from immature pear (Pyrus pyrifolia Nakai) fruit. Food Sci Biotechnol 22:1539-1545. 10.1007/s10068-013-0249-8

Li, J., Zhong, W., Wang, W., Hu, S., Yuan, J., Zhang, B., Hu, T., Song, G. (2014) Ginsenoside metabolite compound K promotes recovery of dextran sulfate sodium-induced colitis and inhibits inflammatory responses by suppressing NF-kB activation. PLoS One 9:e87810. 10.1371/journal.pone.008781024504372PMC3913696

Liu, Y., Wang, X., Hou, Y., Yin, Y., Qiu, Y., Wu, G., Hu, C. A. A. (2017) Roles of amino acids in preventing and treating intestinal diseases: Recent studies with pig models. Amino Acids 49:1277-1291. 10.1007/s00726-017-2450-128616751

Oh, S. Y., Cho, K. A., Kang, J. L., Kim, K. H., Woo, S. Y. (2013) Comparison of experimental mouse models of inflammatory bowel disease. Int J Mol Med 33:333-340. 10.3892/ijmm.2013.156924285285

Okayasu, I., Hatakeyama, S., Yamada, M., Ohkusa, T., Inagaki, Y., Nakaya, R. (1990) A novel method in the induction of reliable experimental acute and chronic ulcerative colitis in mice. Gastroenterology 98:694-702. 10.1016/0016-5085(90)90290-H

Oliveira, M. C., Sichieri, R., Moura, A. S. (2003) Weight loss associated with a daily intake of three apples or three pears among overweight women. Nutrition 19:253-256. 10.1016/S0899-9007(02)00850-X

Oliveira, M. C., Sichieri, R., Mozzer, R. V. (2008) A low-energy-dense diet adding fruit reduces weight and energy intake in women. Appetite 5:291-295. 10.1016/j.appet.2008.03.00118439712

Peng, F., Cheng, C., Xie, Y., Yang, Y. (2015) Optimization of microwave-assisted extraction of phenolic compounds from "Anli" Pear (Pyrus ussuriensis Maxim.). Food Sci Technol Res 21:463-471. 10.3136/fstr.21.463

Qiu, D., Guo, J., Yu, H., Jan, J., Yang, S., Li, X., Zhang, Y., Su, J., Cong, J., He, S., Wei, D., Qin, J. C. (2018) Antioxidant phenolic compounds isolated from wild Pyrus ussuriensis Maxim. Fruit peels and leaves. Food Chem 241:182-187. 10.1016/j.foodchem.2017.08.07228958517

Ramos, G. P., Papadakis, K. A. (2019) Mechanisms of disease: Inflammatory bowel disease. Mayo Clinic Proceeding 94:155-165. 10.1016/j.mayocp.2018.09.01330611442PMC6386158

Randhawa, P. K., Singh, K., Singh, N., Jaggi, A. S. (2014) A review on chemical-induced inflammatory bowel disease models in rodents. Korean J Physiol Pharmacol 18:279-288. 10.4196/kjpp.2014.18.4.27925177159PMC4146629

Song, J. S., Ahn, Y. H. (2002) Phytosociological study on composition, distribution and habitat of the ussurian pear and chinese pear, Korean wild species. Kor J Env Eco 16:160-171.

Sroka, Z., Zgórka, G., Żbikowska, B., Sowa, A., Franiczek, R., Wychowaniec, K., Krzyżanowska, B. (2019) High antimicrobial efficacy, antioxidant activity, and a novel approach to phytochemical analysis of bioactive polyphenols in extracts from leaves of Pyrus communis and Pyrus pyrifolia collected during one vegetative season. Microb Drug Resist 25:582-593. 10.1089/mdr.2018.014930547716

Valatas, V., Bamias, G., Kolios, G. (2015) Experimental colitis models: insights into the pathogenesis of inflammatory bowel disease and translational issues. Eur J Pharmacol 759:253-264. 10.1016/j.ejphar.2015.03.01725814256

Wu, C. C., Chen, J. S., Wu, W. M., Liao, T. N., Chu, P., Lin, S. H., Lin, Y. F. (2005) Myeloperoxidase serves as a marker of oxidative stress during single haemodialysis session using two different biocompatible dialysis membranes. Nephrol Dial Transplant 20:1134-1139. 10.1093/ndt/gfh76415814542

Information

Publisher :Agriculture and Life Sciences Research Institute, Kangwon National University
Publisher(Ko) :강원대학교 농업생명과학대학 농업생명과학연구원
Journal Title :Journal of Agricultural, Life and Environmental Sciences
Journal Title(Ko) :농업생명환경연구
Volume : 33
No :3
Pages :166-175
Received Date : 2021-09-23
Accepted Date : 2021-10-12
DOI :https://doi.org/10.22698/jales.20210017

Journal of Agricultural, Life and Environmental Sciences ISSN:2233-8322(Print) 2508-870X(Online) 농업생명환경연구

All Issue