농업생명환경연구 · 강원대학교 농업생명과학대학 농업생명과학연구원

Research Article

A Study on the Analysis of Consumption Behavior by Expansion of the Import of Dried Fruit: Focused on the Consumers of Dried persimmon in Seoul
건과일 수입확대에 따른 소비행태분석 연구: 서울시의 건(조)감 소비자를 중심으로

Morrison Tay, Jong In Lee
MorrisonTay, 이종인

Increased consumer interest and the formation of new markets are increasing the demand for dried agricultural products. The purpose of the study ... + READ MORE

Increased consumer interest and the formation of new markets are increasing the demand for dried agricultural products. The purpose of the study was to understand the spending habits of 249 dried persimmons consumers. A binary logistic regression model was used to analyze the spending behavior of consumers who preferred domestic dried persimmons versus those who did not, finding that older, single, and higher income and educational background are associated with greater preference for domestic dried persimmons, and the more important they consider quality, storage, nutrition, health, and taste, the more they prefer dried persimmons made in Korea. This study provides useful information for producers of dried persimmons to strengthen their marketing strategy. - COLLAPSE

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July 2021
Research Article

Empirical Analysis of the Effects of the Chinese Soybean Target Price Policy

Emmanuel Amanor Yirenkyi, Jong In Lee

Soybeans play an important role in Chinese agriculture; they are not only the main source of vegetable oil but also an important ... + READ MORE

Soybeans play an important role in Chinese agriculture; they are not only the main source of vegetable oil but also an important source of plant protein. This study examines the Chinese soybean target price subsidy, which is intended to effectively guarantee the basic income of farmers using a dataset containing information on 4 provinces that have implemented such policies as the treatment group and 23 provinces that have not as the control group from the China Rural Statistical Yearbook, an ongoing panel data from the year of 2004 to 2015. A difference-in-difference (DID) model was used to estimate the effect of the soybean target price policy on production and average production, showing that the soybean target price subsidy policy has not solved the problems of soybean production nor fundamentally addressed the issue of soybean reserves. The soybean target price subsidy policy guarantees the basic benefits of farmers, promotes production in the middle and lower levels of soybean culture, and has improved the market pricing mechanism, but there remain such problems as lower target prices, failure to promote farmers’ production, some unreasonable processes in the policiese unreasonab and inadequate subsidies. This research holds important theoretical and realistic significance by serving as a reference for other agricultural product price reforms. - COLLAPSE

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July 2021
Research Article

Analysis of Enzyme Productivity of Halophilic Microorganisms Isolated from Freshwater to Search for Fermentative Strains with Possible Value for Functional Materials Development
기능성 소재 개발용 발효 균주 탐색을 위한 민물에서 분리한 호염성 미생물의 효소 생산능 분석

Kee Yeol Kyong
경기열

This study characterized halophilic microorganisms isolated from freshwater in Korea. Samples were cultivated on marine agar at 37°C for several days ... + READ MORE

This study characterized halophilic microorganisms isolated from freshwater in Korea. Samples were cultivated on marine agar at 37°C for several days. A total of 24 pure colonies obtained from single colony isolates were used for 16S rRNA-based phylogenetic analysis by an ID service located in EzBioCloud database. The obtained 16S rDNA sequences allocated the isolated strains were divided into 3 phyla, 11 families, 12 genera, and 17 species. To confirm whether the isolated strains were candidates for the fermentation of diverse food ingredients and development of functional cosmetic ingredients, amylase, lipase, and protease enzyme assays were performed individually; the tests revealed that 17 strains possessed at least one enzyme activity. In particular, 11 strains exhibited activities of 2 enzymes and 3 strains produced auxin. These strains might be useful in the food and cosmetic industries. Therefore, the study findings will contribute to securing domestic biological resources and improving hydrolytic enzyme activities using isolated strains. - COLLAPSE

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July 2021
Research Article

Isolation and Evaluation of Fungicides for the Control of Alternaria alternata Causing Alternaria Leaf Spot on Aster scaber and Ligularia fischer
곰취 및 참취 점무늬병을 일으키는 Alternaria alternata의 분리 및 살균제 효과 검정

Noh-Hyun Lee, Jong-Hwan Shin, Hee-Yeong Kim, Sa-Heon Kim, Kyoung Su Kim
이노현, 신종환, 김희영, 김사헌, 김경수

As more people have begun paying attention to eating healthy, the value of vegetable crops such as Chwinamul, is increasing. However, various ... + READ MORE

As more people have begun paying attention to eating healthy, the value of vegetable crops such as Chwinamul, is increasing. However, various plant pathogenic fungi cause serious economic losses during the production of Chwinamul but relevant research remains insufficient. In this study, the pathogens responsible for causing leaf spot disease in two cultivars of Chwinamul, Aster scaber, and Ligularia fischer, were isolated and analyzed using the 18S rRNA internal transcribed spacer (ITS) and RNA polymerase II subunit 2 (RPB2) regions to identify the causal pathogens. Two fungal species, Alternaria alternata and Phoma sp., were identified. Based on the abundant isolation of A. alternata from infected tissues of Chwinamul, we re-inoculated A. alternata on A. scaber and confirmed that the pathogen was the main causal agent of leaf spot disease. Metconazole was the most effective fungicide to control A. alternata, showing 100% inhibition of mycelial growth at a concentration of 100 µg/mL. Difenoconazole and cyprodinil were also highly effective with 83.1% and 85.5% inhibition of mycelial growth, respectively, at a concentration of 1,000 µg/mL each. Streptomycin was the least effective, with only 25.3% inhibition at a concentration of 1,000 µg/mL. Our study provides information on the causative agent (A. alternata) of leaf spot disease in Chwinamul and suggests an effective fungicide to combat A. alternata. - COLLAPSE

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July 2021
Research Article

Quality Properties of Hanwoo longissimus Muscle Packaged for Export Market during Long-term Storage at Various Temperatures
한우 수출을 위한 채끝 등심의 온도와 저장 기간에 따른 품질 변화 평가

Moon-ju Kim, Young-dong Cho, In-ho Hwang
김문주, 조영동, 황인호

The current experiment was designed to establish optimal distribution conditions for Hanwoo longissimus lumborum muscle (LL) packaged for a Hong Kong market ... + READ MORE

The current experiment was designed to establish optimal distribution conditions for Hanwoo longissimus lumborum muscle (LL) packaged for a Hong Kong market over 40 days. Eight LL muscles were sampled the day after slaughter from a commercial beef export abattoir located in Seoul. Samples were allocated into a 2 × 4 factorial arrangement (stored at 1°C or 4°C, and aged for 1, 7, 21, or 40 days) and packaged with a 9-layer commercial shrink film; the objective meat quality was determined at the designated days. Moisture content, drip loss, and lipid oxidation data indicated that storage at 1°C was significantly (p < 0.05) more stable than storage at 4°C. In contrast, the Warner-Bratzler shear force showed a similar tendency (p < 0.05) of significant reduction during the aging period. The objective meat color assessed by redness (CIE a*) revealed that storage at 1°C significantly improved (p < 0.05) stage redness up to 40 days, while storage at 4°C maintained a similar redness. The current data indicate that storage temperature is a prime determinant of meat quality, particularly for beef cut packaged in the long-term exporting market, and suggested that maintenance at 1°C is recommended compared to that at 4°C. The current study highlights the crucial need for the development of a cold-chain handling manual for exporting beef. - COLLAPSE

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July 2021
Research Article

Ethylene Treatment and Storage Temperature Affect Ripening Quality of Kiwifruit Cultivars
참다래 품종별 저장 온도와 에틸렌 처리에 따른 품질 변화

Han-Ryul Choi, Hyok Kwon, Shimeles Tilahun, Min-Woo Baek, Hee-Cheol Lee, Do-Su Park, Ki-Seok An, Cheon-Soon Jeong
최한률, 권혁, 시멜레스틸라훈, 백민우, 이희철, 박도수, 안기석, 정천순

In this study, green ‘Hayward’, gold ‘Haegeum’, and red ‘Hongyang’ kiwifruit cultivars were treated with 50 and 100 ppm ethylene in commercial ... + READ MORE

In this study, green ‘Hayward’, gold ‘Haegeum’, and red ‘Hongyang’ kiwifruit cultivars were treated with 50 and 100 ppm ethylene in commercial packing boxes to investigate the postharvest ripening quality during the storage at 10°C and 25°C. As the ripening period proceeded, all three cultivars showed a gradual decreasing trend in firmness and titratable acidity (TA), whereas total soluble solids (TSS) content and Brix acid ratio (BAR) showed an increasing trend. In the case of storage at 25°C, the firmness of ‘Haegeum’ and ‘Hongyang’ ranged from 3.94 to 4.61 N and 4.94 to 5.00 N on the 2nd day at 50 and 100 ppm ethylene, respectively. However, ‘Hayward’ maintained firmness (5.67–7.57 N) up to the 4th day of ripening. In the case of storage at 10°C, there was no significant difference between the control and ethylene treatments. Regarding TSS, all three cultivars showed increasing trends as the ripening period increased, regardless of storage temperature and ethylene concentration, and there was a significant difference between the control and ethylene-treated groups throughout the ripening period at 25°C. The TA of ‘Hayward’ showed the largest difference between the control and treatment as compared to ‘Haegeum’ and ‘Hongyang’ at 25°C. On the 6th day of ripening, the TA of ‘Hayward’ was 1.53 and 0.31 mg/100 g^-1 in the control and 50 ppm ethylene treatment, respectively. In contrast, there was no significant difference between the control and ethylene treatments in ‘Hayward’ and ‘Haegeum’ at 10°C. The BAR showed increasing trends in all three cultivars as the ripening period increased. In particular, ethylene-treated ‘Hayward’ and ‘Haegeum’ showed high BAR values of 35.2 and 44.3, respectively, on the 6th day at 25°C. In addition, firmness, TSS, and TA showed significant correlations (r = －0.54, 0.65, and －0.83, respectively) with BAR. Generally, the effect of ethylene treatment at 10°C was less than at 25°C, which may be due to the low metabolic rates of the fruits at low temperatures. In addition, there was no significant difference in quality with the change in ethylene concentration because the external ethylene could no longer influence the ripening process once fruit ripening was initiated and the internal ethylene concentration reached saturation levels. Taken together, based on firmness, TSS, TA, and BAR, consumers’ preferences for ‘Hayward’ were not met at 10°C until the 6th day of ripening, but at 25°C, consumers’ preferences were attained after the 4th day. In the case of ‘Haegeum’ and ‘Hongyang’, consumers’ preferences were attained starting from the 2nd day of ethylene treatment in both 10°C and 25°C conditions. - COLLAPSE

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July 2021
Research Article

Antagonistic Effects and Biochemical Properties of Bacteria Isolated from Soil
토양에서 분리한 세균의 길항효과 및 생화학적 특성

Hyun Seung Kim, Sang Woo Kim, Mahesh Adhikari, Youn Su Lee
김현승, 김상우, 마헤시아드히카리, 이윤수

This study was conducted to screen the antagonistic efficacy and biochemical characteristics of bacterial isolates from soil. In total, 39 different bacterial ... + READ MORE

This study was conducted to screen the antagonistic efficacy and biochemical characteristics of bacterial isolates from soil. In total, 39 different bacterial strains were isolated from the field rhizospheric soil collected from Bonghwa, Korea in 2017. Serial dilution technique was implied for isolation of bacterial isolates. Results of the in vitro antagonistic screening confirmed that the five bacterial strains have a potential for antagonism. The strains BH9 (Burkholderia sp.), BH29 (Lysinibacillus macroides), BH36 (Paenibacillus peoriae), BH37 (Bacillus subtilis), and BH38 (Burkholderia sp.) are bacterial isolates, antagonistic against different fungal phytopathogens (Alternaria solani, Colletotrichum gloeosporioides, Fusarium oxysporum, Rhizoctonia solani, Sclerotinia minor, and Sclerotinia sclerotiorum) that were obtained from the Korean Agriculture Culture Collection (KACC). The results suggest that BH37 possesses a high range of antagonism against all tested phytopathogenic fungi. However, all five bacterial isolates exhibited significant antagonistic potential; BH37 showed the highest mycelial inhibition of S. sclerotiorum by 95.3%. The five potential antagonistic bacterial isolates were biochemically characterized. Cellulase, chitinase, hydrogen cyanide, and protease production by these isolates were found to be positive in the biochemical characterization assay. Furthermore, highlighting the holistic mechanisms regarding the production of antifungal compounds and lipopeptides by these antagonistic bacterial isolates against phytopathogenic fungi would be worthwhile in the future. - COLLAPSE

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July 2021
Research Article

The Relationship between the Vegetation Index obtained using Unmanned Aerial Vehicles and the Amount of Magnesium in the Soil
무인기를 이용하여 얻은 생육지수와 토양 내 마그네슘 양의 관계

Dong-Wook Kim, Gyung Deok Han, Jae Young Kim, Hak-Jin Kim, Yong Suk Chung
김동욱, 한경덕, 김재영, 김학진, 정용석

Magnesium deficiency in the soil threatens crop production and human health. Despite the trend of reduced rural manpower and increasing urban population ... + READ MORE

Magnesium deficiency in the soil threatens crop production and human health. Despite the trend of reduced rural manpower and increasing urban population consuming more crops, we have to measure and control magnesium levels in the soil for continuous food supply to the population. Using UAV image analysis, the magnesium content in the plants can be measured, providing a valuable clue to predict the magnesium amount in the soil. This study aims to describe the production of an aerial buckwheat field video using UAV. The obtained video was analyzed for the vegetation index (VI) while the buckwheat in the field was sampled and analyzed for the magnesium and chlorophyll contents, as the main building block of chlorophyll is magnesium. The applied VI refers to the Normalized Difference Vegetation Index (NDVI), highly correlating with the chlorophyll content in plants. The results of the regression analysis showed that the NDVI with the total chlorophyll content displays a positive coefficient of R² =0.9546. Moreover, the NDVI with the magnesium content displays a positive coefficient of R² =0.6786 in the buckwheat. In this case study, we present an easy and efficient way to predict the magnesium content of the soil to control soil health. - COLLAPSE

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July 2021
Research Article

Effect of Radiation Treatment on Buckwheat Seed Harvest
방사선 처리가 메밀 종자 수확에 미치는 영향

Jung Min Choi, Gyung Deok Han, Seong Heo, Yong Suk Chung
최정민, 한경덕, 허성, 정용석

The need for crop breeding to cope with population growth and climate change is increasing. Securing various genetic resources would be essential ... + READ MORE

The need for crop breeding to cope with population growth and climate change is increasing. Securing various genetic resources would be essential for solving these near-future problems. However, securing genetic resources in pseudo-cereals, such as buckwheat, has been relatively neglected. Buckwheat is mainly consumed in Asia. In Republic of Korea, approximately 50 % of the consumption is self-sufficient. However, research on buckwheat breeding is limited, and artificial irradiation-related research on buckwheat is also lacking. When exposed to low-dose radiation, atypical in nature, the hormesis effect could be observed in crops.. This study describes the low-dose (10, 15, and 30 Gy) irradiation of buckwheat. As a result, the number of seeds harvested per plant decreased with the low-dose radiation treatment. However, the one-hundred- seed weight was the highest upon the 10 Gy treatment, showing weight gain compared to the control.This result could be used for increasing yield in buckwheat production. - COLLAPSE

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July 2021